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61	Biomedical Science	ABS-94
ADOLESCENT GIRLS NUTRITION ASSISTANCE PROGRAM to PREVENTING STUNTING Tutik Hidayati, Iis Hanifah, Riska Faraswati Stikes Hafshawaty pesantren Zainul Hasan Midwifery Education Profession Study Program, Institute of Health Science Hafshawaty Genggong Street of Educational Area Haf-Sha Islamic Boarding School Zainul Hasan Genggong - Probolinggo, Indonesia Abstract Stunting is one of the nutritional problems in Indonesia. Adolescent girls nutrition intake in Indonesia gifted risk for chronic energy deficiency (CED). If adolescent girls nutrition of wasn^t improve, then in the future would be more and more pregnant womens who have short stature and or CED. This would be impact on increased prevalence Indonesia stunting. Aim of this study analyzed the effect of the nutrition assistance program on healthy food selection adolescent girls knowledge to prevent stunting. Methode pre-experimental, nutrition assistance program as independent variable and healthy food selection knowledge as dependent variabel. Questionnaires as a research tool and data would be analyze with paired-sampel t test. Results showed adolescent girls knowledge increased, it means nutrition assistence program influnced adolescent girls knowledge healthy foods election. That could encourage adolescent girls spirit to contributed in preventing stunting on young women. Keywords: Nutrition assistance Progam, stunting, adolescent girls knowledge Share Link | Plain Format | Corresponding Author (Tutik Hidayati)

62	Biomedical Science	ABS-95
STOP STUNTING: IMPROVING WOMEN^S NUTRITION DURING PREGNANCY WITH EMODEMO METHOD Wahida yuliana 1,a), retno hastri risqi romdani 1,b), bawonul hakim 1,c) 1Departement of Midwifery, Sekolah Tinggi Ilmu Kesehatan Hafshawaty Pesantren Zainul Hasan Probolinggo,Indonesia a)Corresponding author: wahidayuliana44[at]stikeshafshawaty.ac.id b)retnohastri[at]stikeshafshawaty.ac.id c)bawonnulhakim[at]stikeshafshawaty.ac.id Abstract Stunting is a growth problem that occurs in children. This occurs from exposure of the fetus to nutritional deficiencies that result in fetal growth restriction. The critical period during pregnancy for fetal growth and development has led to the focus of the program on these ^1,000 days^ in the life cycle. Dietary interventions, including nutrition education and the provision of dietary supplements to malnourished mothers during pregnancy, result in improved fetal growth positioning the newborn for healthier growth. Emodemo stands for Emotional Demonstration is a method of delivering simple messages with participatory activities that are fun and emotional. Emodemo has several modules, one of which is the ATIKA module, which contains the demonstration of iron sources for pregnant women. This simple message from emodemo invites pregnant women to consume chicken liver, eggs and fish in order to fulfill the nutritional needs of pregnant women. Researchers want to know whether this emodemo method can change the diet of pregnant women. It is hoped that nutritional needs will be fulfilled and reduce one of the factors that cause stunting. The emodemo method is a program implemented in every area of the health facility. This study is an experimental study that will compare areas that are not exposed to the implementation of emodemo with areas that have been programmed to receive emodemo for pregnant women. The data used screening sheets, observation sheets and interviews. Bivariate analysis using chi-square with a significance level of 95% (alpha 0.05). There is a change in the diet of pregnant women by consuming chicken liver, eggs and fish alternately every day so that nutritional needs are fulfilled. This emodemo method is very important to study as a way to stop stunting through adequate prenatal nutrition Keywords: Stunting,nutrition,Emodemo Share Link | Plain Format | Corresponding Author (Wahida Yuliana)

63	Biomedical Science	ABS-98
Determinant Factors of Hypertension in The Elderly Nur Hamim1,a, Nafolion Nur Rahmat 2,b, Tutik Ekasari 3,c 1Departement of Nursing, Sekolah Tinggi Ilmu Kesehatan Hafshawaty Pesantren Zainul Hasan, Probolinggo East Java, Indonesia. 2Departement of midwifery, Sekolah Tinggi Ilmu Kesehatan Hafshawaty Pesantren Zainul Hasan, Probolinggo East Java, Indonesia Abstract Hypertension is still a major public health problem, especially in developing countries. Elderly as a special group that is susceptible to degenerative diseases, which is influenced by many factors including the behavior of awareness of the importance of maintaining health which is still lacking so that they experience hypertension. Therefore hypertension is a risk factor for cardiovascular disease. Which suffers a lot by the elderly. This study aims to analyze the risk factors for hypertension in the elderly over 50 years of age.The method in this study is a survey approach, designed to determine the determinants of hypertension, especially in the elderly. While the observational design was used to examine the risk factors for hypertension in the age group above 50 years with a cross-sectional approach. The sampling technique used purposive sampling. There are 61 respondents. Collecting data using a questionnaire and observing the variables of blood pressure and anthropometry in each respondent. The results showed that the prevalence of hypertension sufferers in the sample population was 71.6% (male 70.5%, female 72.7%). Adherence to treatment is 19 percent, level of awareness is 30 percent, and care for the elderly with hypertension is 21 percent. The results of logistic regression analysis showed that the prevalence of hypertension was associated with the Madurese population, obesity, stroke and with outpatient care. Thus, the prevalence in the elderly group is still quite high which puts them at risk for cardiovascular disease. The low level of self-care, level of awareness and treatment are strong reasons for the need for continuous health education, social and family support for the elderly in managing hypertension. Keywords: Determinant Factors, Hypertension, Elderly Share Link | Plain Format | Corresponding Author (Nur Hamim)

64	Biomedical Science	ABS-107
The Comparison of TGF-B1, Slug, and Twist Gene Expression in Colorectal Subjects with Hepatic and non-Hepatic Metastatic Adinda Permatasari1, Ikke Astrid Dewi1, Rifqah Dwi Pratiwi 2, Hendra Susanto1, a), Adeodatus Yuda Handaya3, 4, b), and Moch Sholeh1 1Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, Indonesia 2Department of Biotechnology., Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang Indonesia 3Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia 4Division of Digestive Surgery, Department of Surgery, Dr. Sardjito Hospital, Yogyakarta, Indonesia a) Corresponding author: hendrabio[at]um.ac.id b) yudahandaya[at]ugm.ac.id Abstract In 2020, GLOBOCAN reported that the incidence of Colorectal Cancer (CRC) in men was in third place and in women it was in second. CRC can be caused by genetic factors and environmental factors. Epidemiological studies suggest an unhealthy lifestyle can be a strong candidate for the development of CRC. The development and metastasis of CRC are characterized by the epithelial-mesenchymal transition (EMT). There are many genes known to be involved in CRC malignancy, including TGF-B1, Slug and Twist. This study aims to determine the differences in TGF-B1, Slug and Twist gene expression in CRC patient with and without liver metastasis. This research was carried out by measuring the expression of all genes using the RT-qPCR method. Importantly, the expression of slug genes in non-hepatic metastasis patients was higher than other patient groups. TGF-B1, Slug and Twist is potential to be used in treatment strategies or predictions for CRC patients related to the significant difference the expression in the two groups of patients. Hence, the development of CRC gene biomarker for early stages detection can be focused on the primary gene regulator-linked metastasis. Keywords: CRC Hepatic Metastatic- CRC non-Hepatic Metastatic- TGF-B1- Slug- Twist Share Link | Plain Format | Corresponding Author (Adinda Permatasari)

65	Biomedical Science	ABS-120
Cytotoxicity Evaluation of Urena Lobata Leaf Extract and Their Fraction on Raw 264.7 Cells Using Tetrazolium (MTT) Yudi Purnomo1, a), Doti Wahyuningsih2, Andri Tilaqza1 1 Pharmacy Department, Medical Faculty, University of Islam Malang, Malang, Indonesia 2 Biochemistry Department, Medical Faculty, University of Islam Malang, Malang, Indonesia Abstract Pulutan (Urena lobata) is herbal medicine that used to treat some diseases related to inflammation condition. Pre-clinical studies showed its efficacy, however, the study about their safety have not been evaluated completely. The objective of study to determine toxicity level of Urena lobata (U.lobata) leaf extract and their fraction on RAW 264.7 cells using tetrazolium (MTT) assay. U. lobata leaf was extracted by digestion methods using ethanol solvent, therefore, the extract was fractionated by n-hexane (fraction A), ethyl acetate (fraction B), n-buthanol (fraction C) and water (fraction D) respectively. Extract and fractions are tested on RAW 264.7 cells, furthermore percentage viability values of the cells determined by the tetrazolium salt reduction assay (MTT). The half-maximal inhibitory concentrations (IC50) of viability cells were determined (n=3) for at least four different concentrations. Fraction A showed inhibition on viability cells (IC50 = 2941,73 ppm) stronger than fraction B (IC50 = 3967,76 ppm), fraction C (IC50 = 12473,33 ppm) and fraction D (IC50 = 19048,46 ppm). Meanwhile ethanolic extract (IC50 = 7515,63 ppm) lower than fraction A and fraction B but stronger than fraction C and D to inhibit viability cells. Fraction A of U.lobata has toxicity level strongest among fraction B, C, D and its extract. Keywords: cytotoxicity, raw 264.7 cell, urena lobata, tetrazolium Share Link | Plain Format | Corresponding Author (Yudi Purnomo)

66	Biomedical Science	ABS-131
The Relationship Between 25-Hydroxyvitamin D (25[OH]D) Levels with Serum Levels of Interleukin-4 (IL-4) and Interleukin-10 (IL-10) in Children with Mild and Moderate Asthma Ade Habibi (1a), Wisnu Barlianto (1b) 1)Department of Child Health, Faculty of Medicine, University of Brawijaya, Indonesia a) dr.adehabibi[at]gmail.com b) wisnu_barlian[at]yahoo.com Abstract Asthma is a respiratory disease associated with chronic inflammation, hyperresponsiveness, and airway obstruction. Various cytokines may play a role in the pathophysiology of asthma, such as Th 2 cytokines (IL-4, IL-3, IL-5, and IL-13), which increase eosinophils and IgE levels that cause vasodilation, bronchial smooth muscle contraction, and mucus secretion, whereas IL-10 prevents chronic inflammation and tissue damage. Several hypotheses about nutrition are associated with asthma, including vitamin D levels, but this hypothesis is debatable. This research is cross-sectional, using consecutive sampling with 30 participants. Based on the relationship between vitamin D levels and average serum IL-4 levels, the groups with vitamin D deficiency, insufficiency, and sufficiency were 20.26 pg/ml, 2.08 pg/ml, and 1.89 pg/ml, respectively. Meanwhile, the average levels of IL-10 based on groups with vitamin D deficiency, insufficiency, and sufficiency were 5.14 pg/ml, 8.38 pg/ml, and 13.79 pg/ml, respectively. The results concluded that 25(OH)D levels have a significant negative relationship with serum IL-4 levels and a significant positive relationship with serum IL-10 levels in children with mild and moderate asthma. Keywords: 25-Hydroxyvitamin D (25(Oh)D) Levels- Interleukin-4 (IL-4)- Interleukin-10 (IL-10)- Mild and Moderate Asthma. Share Link | Plain Format | Corresponding Author (Ade Habibi)

67	Biomedical Science	ABS-138
The effect of dimefluthrin-based mosquito repellent activity on histopathological findings in the spleen and pancreas Ary Andini, Asma Rubiah Rosyadahan, Shinta Nuryah Azizah, Devyana Dyah Wulandari, Ersalina Nidianti Department of Medical Technology Laboratory, Faculty of Healthy, Universitas Nahdlatul Ulama Surabaya, Jl. Jemursari No. 51-57 Surabaya, East Java, Indonesia Abstract Mosquito repellent contains active substances that can prevent and paralyze mosquito activity. One of the active substances that play a role is dimefluthrin which has a negative effect on health if used for a long time. This study aims to analyze the effect of exposure to insect repellent on histopathological findings in the spleen and pancreas of Wistar rats. The study used Wistar rats which were divided into 4 groups, namely the control group and the intervention group including mosquito coils (dimefluthrin 0.031%), mosquito repellent liquid (dimefluthrin 0.014%), and insect repellent mat (dimefluthrin 0.566%) which were exposed for 8 hours in 20 days, respectively. Histopathological findings in the spleen and pancreas due to exposure to insect repellent seen in the electric mosquito repellent mat have the highest scores in cell degeneration and necrosis. The higher dimefluthrin content in insect repellent can affect the condition of the spleen and pancreas. Keywords: mosquito- repellent- dimefluthrin- spleen- pancreas Share Link | Plain Format | Corresponding Author (Ary Andini)

68	Biomedical Science	ABS-154
The Effects of Paraquat Dichloride Exposure on Lung Histology of Mice (Mus musculus) Through Nebulizer Inhalation Method Daffa^ Rizal Dzulfaqaar Alauddin1, Iin Lailatul Khoirunnisa^1, Hani Dwi Siswandini1, Rifqi Hilman1, Diajeng Ravika Adil Bangun Negara1, Sholiha Azizatul Zahro1, and Yunita Rakhmawati1, a) Universitas Negeri Malang Abstract Paraquat dichloride is one of the active ingredients in the Gramoxone herbicide, which farmers use to control weed growth in crops. The widespread use of the herbicide paraquat raises serious concerns about pollution hazards, one of which is health-related. The herbicide paraquat dichloride has the potential to cause lung disorders such as pulmonary fibrosis. This study aimed to determine the effect of exposure to paraquat dichloride on changes in behavior and lung histology of male mice (Mus musculus) due to the formation of scar tissue and thickening of the alveolar walls through the inhalation method. In this experimental study, 24 male mice (Mus musculus) were randomly divided using a completely randomized design) into two groups, namely the control and treatment groups. The treatment group was given the herbicide paraquat dichloride of 50 mg/kg BW dissolved in 1 ml of distilled water for 13 days by inhalation method using a nebulizer. During the study, it was found that behavioral changes in mice that had been treated, such as changes in behavior, were less active, decreased appetite, increased respiratory frequency, and there were inconsistent body weights in mice compared to the control group. Keywords: inhalation method, mice, paraquat dichloride, pulmonary fibrosis Share Link | Plain Format | Corresponding Author (Luthfi Syarifa Rasyidatu Hasnanda)

69	Biomedical Science	ABS-159
The Potential of purple Dioscorea alata L. Bulbs as a Retinopathy therapy on the Histological Structure of Retina Layer on Type 1 Diabetes Mellitus Zebrafish model Anita Restu Puji Raharjeng, Juniman Rey, Purnonomo, Nastiti Wijiayanti, Bambang Retnoaji, Nur Indah Septriani Biology Faculty, Universitas Gadjah Mada Abstract Diabetic retinopathy is the most specific vascular complication of diabetes mellitus. The cause of diabetic retinopathy is the presence of angiogenesis. Angiogenesis is induced by activation of the Vascular Endothelial Growth Factor (VEGF). The content of Quercetin, one of the flavonoids in uwi Tuber (Dioscorea alata) can inhibit VEGF. This study aimed to determine the histological structure of the eye and the angiogenic activity of uwi tuber (Dioscorea alata) in the retina of zebrafish (Danio rerio) with type 1 diabetes mellitus induced by Streptozotocin. This study used a True Experimental Laboratory through the Randomized Posttest Only Controlled Group Design method with Danio rerio which was divided into 4 groups, namely 1). the negative control group (K-) which was only given a standard commercial fish food diet, 2). TDU is the normal healthy fish that was fed with D. alata, 3). The positive control group (K+) was the diabetic fish that had already been treated with STZ at a dose of 0.003g/ml by immersion method, and was only given a standard commercial fish food diet, 4). The STZDu group is the diabetic fish (treated with STZ) and was fed with D. alata (0.2mg/g BW of Dioscorea alata, 3 times feeds per day). The results showed that there are five layers on the zebrafish retina, GCL, IPL, INL, ONL, and RPE, the histological structure of zebrafish^s retina layer with DM on GCL, IPL, ONL, and RPE is significantly thinner than normal, while the INL is significantly thicker than normal. From a physiological perspective of zebrafish with DM, the thickness and thinness were significantly associated with visual field deviation and optic disc hemorrhage. The survival rate of the D. rerio is decreasing over time because the fish feel stressed, and or death occurs after checking blood sugar. The condition of hyperglycemia in fish decreased after receiving D. alata as a feed treatment, but continuous consumption of D. alata could trigger hypoglycemia in D. rerio. Keywords: Angiogenetic, Danio rerio, Diabetes Mellitus, Dioscorea alata, Retinopathy. Share Link | Plain Format | Corresponding Author (Anita Restu Puji Raharjeng)

70	Bionanotechnology	ABS-3
GREEN SYNTHESIS OF SILVER NANOPARTICLES USING ALGAES (Fucus vesiculosus, Euchema spinosus and Gracillaria verrucosa) as ANTIOXIDANTS AND ANTICOLLAGENASE Evika Sandi Savitri*, Shinta, Eko Budi Minarno Department Biology Universitas Islam Negeri Maulana Malik Ibrahim Malang Jl. Gajayana No 50 Malang Abstract The green synthesis of silver nanoparticles is an environmentally friendly method to obtain compounds that are useful as anti-aging candidates. The algae extract used contains secondary metabolites such as terpenoids and flavonoids, hence it acts as a bio-reductant agent for the production of silver nanoparticles. This research aims to synthesize silver nanoparticle using algae, antioxidant activity test and anticollagenase enzyme activities among 3 species of algae are Fucus vesiculosus, Euchema spinosus, and Gracillaria verrucosa. The silver nanoparticle character test was performed using a Particle Size Analyzer (PSA), Scanning Electron Microscope (SEM), and X-ray Diffraction (XRD). Furthermore, the antioxidant test was conducted using the DPPH method while the anticollagenase activity test was performed using spectrophotometry with standard collagenase. The silver nanoparticles produced from Fucus vesiculosus extract had a particle distribution with a size of 74.06 nm. Also, the particle size distribution from the extract of Euchema spinosum ranges in sizes from 53.70 nm to 100 nm. Meanwhile, the particle size distribution from the extract of Gracilaria spp has a size of 123.4 nm. The SEM imaging results showed that the obtained ZnO was generally spherical in shape, but the gap between the particles was not visible. Therefore, the antioxidant and anticollagenase enzyme activity test results showed that Fucus vesiculosus > Euchema spinosum > Gracillaria verrucosa, respectively. Keywords: green synthetis, silver nanoparticles, algae, anti-aging Share Link | Plain Format | Corresponding Author (Evika Sandi Savitri)

71	Bionanotechnology	ABS-29
Fourier Transform Infrared Spectroscopy Characterization and Antioxidant Activities of Single Garlic Extract-loaded Chitosan-Alginate Nanoencapsulation Nadiya Dini Rifqi (a), Sri Rahayu Lestrari (a*), Abdul Gofur (a), Sunaryono (b) a) Department of Biology, Faculty of Mathematics and Natural Science, Universitas Negeri Malang, Jl. Semarang 5, Malang 65145, Indonesia *srirahayulestari[at]um.ac.id b) Department of Physics, Faculty of Mathematics and Natural Science, Universitas Negeri Malang, Jl. Semarang 5, Malang 65145, Indonesia Abstract Single garlic extract (SGE) can be chosen as an antioxidant source. Antioxidants are able to reduce the negative effects of free radicals that trigger various types of diseases. SGE has some weaknesses in its utilization. Nanotechnology such as chitosan-alginate nanoencapsulation (CANe) is expected to cover the weaknesses of SGE. The nanoencapsulation of single garlic extract (SGE-CANe) were tested by fourier transform infrared spectrometry (FTIR) to obtain chemical characteristics, functional groups before and after nanoencapsulation is applied. Data collection of antioxidant activity of SGE and SGE-CANe were obtained using the DPPH assay method. The FTIR results show the SGE IR graph has amine, hydroxyl, carbonyl, carboxylate and organosulfur as its functional groups. CANe added amine (C-N), carboxylate (COO-), amide (N-H), and amine and hydroxyl strains (N-H and O-H). The results of the DPPH assay showed the IC50 value of SGE was 19312,5 ppm and the IC50 value of the SGE-CANe results was 32670 ppm. CANe reduces the antioxidant activity of the single active compound of garlic. Overall, this SGE-CANe need a further development so that it has potential as a promising therapeutic agent and supports the benefits of SGE. Keywords: Nanoencapsulation- Chitosan-alginate- Single garlic- FTIR- Antioxidant Share Link | Plain Format | Corresponding Author (Nadiya Dini Rifqi)

72	Bionanotechnology	ABS-30
Characterization and Hemocompatibility Assay of In-Vitro Single Garlic Extract-loaded Chitosan-Alginate Nanoencapsulation Amalia Nur Rahma (a), Sri Rahayu Lestari (a*), Abdul Gofur (a), Siti Imroatul Maslikah (a) a) Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, Jl. Semarang 5, Malang 65145, Indonesia *srirahayulestari[at]um.ac.id Abstract Single garlic is a tropical plant with high bioactive compounds. Most bioactive compounds were found in single garlic is allicin but has low bioavailability. Allicin was classified to Reactive Sulfur Species (RSS) and has the ability to damage eukaryotic cells, for example is red blood cells. The damaged of red blood cells was minimized with a specific mechanism using the Drug Delivery System (DDS) with Chitosan-Alginate Nanoencapsulation (CANe). The aim of this study is to characterization and hemocompatibility assay of red blood cells. Single garlic extraction method using maceration with ethanol 70%. Optimal formulation of SGE-CANe determined using characterization with Particle Size Analyzer (PSA) and hemocompatibility assay. PSA of SGE-CANe such as Z-Average (390,540 nm), Polydispersity Index (PDI) (0,609), and zeta potential (-23,067 mV) shows that SGE-CANe categorized into optimal DDS. Hemocompatibility assay shows that SGE-CANe has low hemolysis percentage than SGE. The result of hemolysis percentage SGE-CANe does not cause the damage of red blood cells. Thus, it can be concluded that SGE-CANe was optimal increasing bioavailability of allicin compounds, hence SGE-CANe was compatible with red blood cells. Keywords: SGE-CANe- Hemocompatibility- Single garlic Share Link | Plain Format | Corresponding Author (Amalia Nur Rahma)

73	Bionanotechnology	ABS-31
Morphological Characterization and Antioxidant Activity Test of Single Garlic Extract-loaded Chitosan-Alginate Nanoencapsulation Ikfi Nihayatul Mufidah (a), Sri Rahayu Lestari (a*), Siti Imroatul Maslikah (a), Sunaryono (b) a) Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, Jl. Semarang 5, Malang 65145, Indonesia *srirahayulestari[at]um.ac.id b) Department of Physics, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, Jl. Semarang 5, Malang 65145, Indonesia Abstract Single garlic is often used in traditional medicine. The compounds include organosulfur and phenolic which have antioxidant activity. Allicin is a major component of antioxidant compounds, but it is unstable and easily degraded in the digestive. Chitosan-alginate nanoencapsulation promises to protect allicin from being degraded, so its antioxidant activity is more optimal in the body. The purpose of this study was to characterize the morphology of single garlic extract-loaded chitosan-alginate nanoencapsulation (SGE-CANe) and to examine the effect of chitosan-alginate nanoencapsulation (CANe) on the antioxidant activity of single garlic. The research procedure includes single garlic extraction, preparation of SGE-CANe, morphological of SGE-CANe, and antioxidant activity test. The morphological characterization of SGE-CANe was using SEM, while the antioxidant activity was using the DPPH test. The results showed that the morphology of SGE-CANe was spherical with a size of < 500 nm. The results of the antioxidant activity test showed that the CANe had an effect on the antioxidant activity of single garlic. The antioxidant activity of SGE-CANe at 24 000 ppm was 65%, while the antioxidant activity of SGE was 52%. CANe protects antioxidant compounds of single garlic extract so that antioxidant activity is more optimal. Keywords: Single garlic- Allicin- Chitosan-alginate nanoencapsulation- Morphology, Antioxidant activity Share Link | Plain Format | Corresponding Author (Ikfi Nihayatul Mufidah)

74	Bionanotechnology	ABS-34
Characterization and Effectiveness Test of Single Garlic Extract-loaded Chitosan-Alginate Nanoencapsulation to Hemolysis Percentage Dahniar Nur Aisyah (a), Sri Rahayu Lestari (a*), Siti Imroatul Maslikah (a), Sunaryono (b) a) Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, Jl. Semarang 5, Malang 65145, Indonesia *srirahayulestari[at]um.ac.id b) Department of Physics, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, Jl. Semarang 5, Malang 65145, Indonesia Abstract Single garlic grows as a result of an unsuitable growing environment. Single garlic has higher active compound content than normal cloves of garlic. One of the active compounds is allicin. Allicin causes hemolysis when in the bloodstream. Chitosan-alginate nanoencapsulation (CANe) is a delivery system that is able to protect the active compounds in single garlic extract (SGE), so it is suspected that it can minimize the degradation of the active compounds and also reduce the hemolysis. This study aims are for characterizing and test the effectiveness of SGE-CANe on the hemolysis percentage. This research method were single garlic extraction, preparation of SGE-CANe, characterization of SGA-CANe (particle size, polydispersity index, zeta potential), and in-vitro SGE-CANe hemocompatibility test using the blood of Balb/C mice. The results showed that the SGE-CANe droplet size was 438,6 nm- SGE-CANe polydispersity index (PdI) value was 0,446- the zeta potential of SGE-CANe was -24,5 mV- and the hemolysis percentage of SGE-CANe (3,05%) < SGE (8,95%). The particle size and PdI of SGE-CANe have reached the research criteria, which is nanometer-sized particles and homogeneously distributed, but the zeta potential value is low. The CANe formulation reduced the hemolysis percentage of SGE in the blood of Balb/C mice. Keywords: Balb/C mice- Characterization- Chitosan-alginate nanoencapsulation- Hemolysis percentage- Single garlic extract Share Link | Plain Format | Corresponding Author (Dahniar Nur Aisyah)

75	Bionanotechnology	ABS-41
PHYTOCHEMICAL SCREENING AND SYNTHESIS NANOPARTICLES OF MOJO LEAF EXTRACT (Aegle marmelos) FOR BOTANI PESTICIDES Sofia Ery Rahayua), Fatchur Rohman, Rahmi Masita, and Bagus Priambodo Department of Biology, Faculty of Mathematics and Natural Science, Universitas Negeri Malang, Indonesia Abstract The purpose of the study was to determine the chemical composition and synthesis of nanoparticles based of maja (Aegle marmelos) leaf extract. Maja leaves were obtained from Malang, Indonesia. Maja leaf extract was made with methanol and chloroform as solvents. Phytochemical screening of maja leaf extract was analyzed by LCMS. The synthesis nanoparticles of maja leaf extract using chitosan as a coating. The synthesis was carried out by 2 methods without and using ionic gelation. Measurement of nanoparticles was carried out using the Particle Size Analyzer (PSA) at the ILRC Laboratory of the University Indonesia. The results of the study found 108 active compounds in the methanol extract and 92 compounds in the chloroform extract of maja leaves. The total phenol of the maja leaf extract was 2.47 mg/ml and the total flavonoid was 12.31 mg/ml. Synthesis of nanoparticles without using ionic gelation resulted in a polydispersity was 1.7, while using ionic gelation was < 1. Both methods produced particle sizes 800 nm and zeta potential values >30 were quite stable. Based on the results of this study, the compound mixing process is better by adding ionic gelation with sonication because it produces good particle uniformity and is quite stable. Keywords: mojo (Aegle marmelos) leaf extract, nanoparticles, chitosan, phytochemical screening Share Link | Plain Format | Corresponding Author (Sofia Ery Rahayu)

76	Bionanotechnology	ABS-45
The Effect of Nanoparticles Allium sativum, Curcuma mangga, and Acorus calamus on Profile of Mice Leukocytes that Induced by Cisplatin Farah Fauzia Maulahibati, Bayyinatul Muchtaromah, and Mujahidin Ahmad Department of Biology, Faculty of Science and Technology, The State Islamic University of Maulana Malik Ibrahim Malang, Jl. Gajayana 50, Malang, Indonesia Abstract Abstract. The induction of cisplatin can increase the production of reactive oxygen species (ROS) and cause an imbalance in the immune system. This condition can be overcome with use of A. sativum, C. mangga, and A. calamus. That plants contain several active compounds to improve the function of the immune system, including leukocytes. This study aimed to know the effect of nanoparticles A. sativum, C. mangga, and A. calamus on the total cell leukocyte and total leukocyte types (neutrophil, basophil, eosinophil, lymphocyte, and monocyte) in mice that induced by cisplatin. This study used the Complete Random Design (CRD) with 5 treatments and 6 replicates. The examination of the total leukocyte used a hemocytometer and the total leukocyte types used a blood smear. The results showed that there was an effect of giving nanoparticles A. sativum, C. mangga, and A. calamus on the total leukocyte, neutrophils, and lymphocytes mice and there was no effect of giving nanoparticles A. sativum, C. mangga, and A. calamus to basophils and eosinophils mice that are induced by cisplatin. The dose of nanoparticles extracts A. sativum, C. mangga, and A. calamus 25 mg/kgBW and 50 mg/kgBW had a significant effect compared to the other treatment groups. Keywords: Acorus calamus, Allium sativum, Curcuma mangga, Leukocyte, Nanoparticle Share Link | Plain Format | Corresponding Author (Farah Fauzia Maulahibati)

77	Bionanotechnology	ABS-63
The Effect of Allium sativum, Curcuma mangga, and Acorus calamus Nanoparticles to Profile of Mice Leukocytes Induced by Cisplatin Bayyinatul Muchtaromah (a*), Farah Fauzia Maulahibati (a), Prilya Dewi Fitriasari (a), Mujahidin Ahmad (a), Eko Budi Minarno (a), and Kenji Mishima (b) (a) Department of Biology, Faculty of Science and Technology, The State Islamic University of Maulana Malik Ibrahim Malang, Jl. Gajayana 50, Malang, Indonesia *bayyinatul[at]bio.uin-malang.ac.id (b) Department of Chemical Engineering, Faculty of Engineering, Fukuoka University, Japan Abstract The induction of cisplatin can increase the production of reactive oxygen species (ROS) and cause an imbalance in the immune system. This condition can be overcome with use of A. sativum, C. mangga, and A. calamus. That plants contain several active compounds to improve the function of the immune system, including leukocytes. This study aimed to know the effect of nanoparticles A. sativum, C. mangga, and A. calamus on the total cell leukocyte and total leukocyte types (neutrophil, basophil, eosinophil, lymphocyte, and monocyte) in mice that induced by cisplatin. This study used the Complete Random Design (CRD) with 5 treatments and 6 replicates. The examination of the total leukocyte used a hemocytometer and the total leukocyte types used a blood smear. The results showed that there was an effect of giving nanoparticles A. sativum, C. mangga, and A. calamus on the total leukocyte, neutrophils, and lymphocytes mice and there was no effect of giving nanoparticles A. sativum, C. mangga, and A. calamus to basophils and eosinophils mice that are induced by cisplatin. The dose of nanoparticles extracts A. sativum, C. mangga, and A. calamus 25 mg/kgBW and 50 mg/kgBW had a significant effect compared to the other treatment groups. Keywords: Acorus calamus, Allium sativum, Cisplatin, Curcuma mangga, Leukocytes, Nanoparticles Share Link | Plain Format | Corresponding Author (Bayyinatul Muchtaromah)

78	Bionanotechnology	ABS-69
In-vitro toxicity study of Chitosan/Alginate nano-encapsulated single garlic extract on 3T3-L1 cell Sri Rahayu Lestari (1*), Hartatiek (2), Abdul Gofur (1), Yuslinda Annisa (1), Nik Ahmad Nizam Nik Malek (3,4) (1) Department of Biology, Faculty Mathematics and Natural Science, Universitas Negeri Malang, Malang, East Java, Indonesia *srirahayulestari[at]um.ac.id (2) Department of Physics, Faculty Mathematics and Natural Science, Universitas Negeri Malang, Malang, East Java, Indonesia (3) Department of Biosciences, Faculty of Science, Universiti Teknologi Malaysia, 81310 Johor Bahru, Malaysia (4) Center for Sustainable Nanomaterials, Ibnu Sina Institute for Scientific and Industrial Research, Universiti Teknologi Malaysia, 81310 Johor Bahru, Malaysia Abstract Single garlic (Allium sativum) is one of the local plants that is used as a medicinal ingredient. Single garlic^s active compounds such as Allicin are easily degraded in the digestive tract, so the expected health effects are not optimal. The encapsulation process can increase the solubility and bioavailability of active compounds in single garlic. Chitosan and alginate are natural biopolymers that can be used as nanoparticle candidates for encapsulation of single garlic active compounds because they are not toxic. This study aims to formulate and test the cytotoxicity of chitosan/alginate nano encapsulated single garlic extract (CAN-SGE) in vitro. The methods used include single garlic extraction with ethanol solvent, CAN-SGE formulation and characterization, and CAN-SGE toxicity test. Characterization of CAN-SGE using Particle Size Analyzer (PSA). A cytotoxicity test was carried out using the MTT assay method. The results showed that the mean droplet size of CAN-SGE was 603,5 nm, the polydispersity index (PDI) was 0,547 and the Zeta potential was -15,7 mV. CAN-SGE has low toxicity at concentrations below 1000 ppm with a cell viability value of 88%. Chitosan/alginate nano encapsulated has the potential to be a drug delivery system for single garlic extract. Keywords: Single Garlic- Chitosan- Alginate- Nanoencapsulation- Toxicity Share Link | Plain Format | Corresponding Author (Sri Rahyu Lestari)

79	Bionanotechnology	ABS-87
Synthesis and Characterization of ZnO Nanoparticles with Biostabilizers Soursop Leaves (Annona muricata L) Using Green Synthesis Method Siti Imroatul Maslikah1, a), Sri Rahayu Lestari1, Awil Endar Pramesti1, Aisy Hanifah1, Rizqi Zidni Hidayati2 1Biology Department, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, Indonesia 2Biology Department, Faculty of Mathematics and Natural Sciences, Universitas Brawijaya Malang, Indonesia a)Corresponding author: siti.imroatul.fmipa[at]um.ac.id Abstract Abstract. Soursop leaf (Annona muricata L) is used as a biostabilizer because it contains phenolic compounds in the form of alkaloids, flavonoid tannins, saponins, steroids and triterpenoids. This research focuses on the synthesis and characterization of ZnO nanoparticles using ZnSO4 and soursop leaf extract as biostabilizer. The purpose of this study was to determine the potential of soursop leaf (Annona muricata L) as a biostabilizer for the synthesis and characterization of ZnO nanoparticles using the green synthesis method. The method used is by incubating at a temperature of 300C between soursop leaf extract and ZnSO4 so that nanoparticle deposits are formed. ZnO nanoparticle characterization using FTIR, XRD, SEM-EDX. The results of FTIR characterization showed that there were three stretches of ZnO, namely (421-476 cm-1), (648-673 cm-1), (829-835 cm-1). Results Based on the XRD characterization, the value of 2&#952- showed the peak of ZnO crystallinity while the SEM test showed nanoparticle sizes of 84.74nm, 86.52nm, 88.07nm and 89.88nm, while the results of EDX characterization contained impurities other than Zn and O elements but with a small percentage. Keywords: Green synthesis, Annona muricata, ZnO nanoparticles, characterization Keywords: Green synthesis, Annona muricata, ZnO nanoparticles, characterization Share Link | Plain Format | Corresponding Author (Siti Imroatul Maslikah)

80	Bionanotechnology	ABS-119
Green Biosynthesized Silver Nanoparticles using Ficus deltoidea Leaf Aqueous Extract as an Antibacterial Agent Ibrahirul Qamil Ismail (a), Nik Ahmad Nizam Nik Malek (a*,b), Hariz Asraf Hassan (a), and Shahrulnizahana Mohammad Din (a) a) Department of Biosciences, Faculty of Science, Universiti Teknologi Malaysia (UTM), 81310 UTM Johor, Malaysia *niknizam[at]utm.my b) Centre for Sustainable Nanomaterials (CSNano), Ibnu Sina Institute for Scientific and Industrial Research (ISI-ISIR), Universiti Teknologi Malaysia (UTM), 81310 UTM Johor, Malaysia Abstract This study aimed to synthesize silver nanoparticles (AgNPs) using a greener synthesis, utilizing Ficus deltoidea plant extract as a reducing and stabilizing agent that is capable for inhibiting bacterial growth. A preliminary study was conducted to analyze the antioxidant activity of the leaf plant extract by determining its total phenolic and flavonoid contents. Before synthesizing the AgNPs, the synthesis process was optimized with different parameters to ensure the highest possible yield of AgNPs including different leaf extract concentrations, pH, volume, synthesis temperature, and reaction time. The optimal synthesis conditions were used to synthesize AgNPs and were subjected for stability analysis and characterization. The formation of AgNPs was monitored by using UV-vis spectroscopy. Next, the biosynthesized AgNPs were characterized by using Fourier transform infrared spectroscopy (FTIR) and compared with colloidal AgNPs with plant extract (FdAgNPs) and the unmodified plant extract (FdEx). The extract obtained the most prominent protein peaks at 3400, 1600, and 1360 cm1, which acts as a reducing agent and helps reduce the silver ions to AgNPs. Further characterization of the biosynthesized AgNPs was conducted using X-ray diffraction (XRD) to determine their crystal latices and structure. Peaks obtained at 38, 46, 64, and 76 in AgNPs corresponding to (111), (231), (220), and (311) sets of lattice planes, which are closely connected with the for Ag. The disc diffusion technique (DDT) for antibacterial assay of the AgNPs showed significant formation of halo-zone for Gram-Negative (Escherichia coli) and Gram-Positive (Staphylococcus aureus). It can be concluded that the green synthesis of AgNPs using F. deltoidea plant extract exhibits antibacterial effects against wide spectrum of bacteria (Gram-Negative and Gram-Positive). Keywords: Green Synthesis, Silver Nanoparticles, Ficus deltoidea, Antibacterial Share Link | Plain Format | Corresponding Author (Ibrahirul Qamil Ismail)

81	Bionanotechnology	ABS-139
The Characterization and Stability Performance Test of GAD65 Nanokit for Early Detection of Diabetes Mellitus in Different Ethnics Aulanni^am Aulani^am4,5, Hendra Susanto1,2 a), Dyah Kinasih Wuragil4,5), Ahmad Taufiq3, and Nik Ahmad Nizam Nik Malek6,7 1Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, Indonesia 2Department of Biotechnology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, Indonesia 3Department of Physics, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, Indonesia 4Department of Chemistry, Faculty of Sciences, Universitas Brawijaya, Indonesia 5Faculty of Veterinary Medicine, Universitas Brawijaya, Indonesia 6Department of Biosciences, Faculty of Science, Universiti Teknologi Malaysia (UTM), 81310 UTM Johor, Malaysia 7Centre for Sustainable Nanomaterials (CSNano), Ibnu Sina Institute for Scientific and Industrial Research (ISI-ISIR), Universiti Teknologi Malaysia (UTM), 81310 UTM Johor, Malaysia Abstract Diabetes mellitus is a chronic disease with a higher cost worldwide. Type 1 diabetes mellitus (T1DM) is an autoimmune disease associated with beta-cell degeneration. Consequently, it will progress to insulin-dependent conditions for the patients. GAD65 was established as the potential early biomarker for beta-cell dysfunction in the initial step of TIDM development. Therefore, this study focused on developing and producing GAD65 kits to become the diagnostic tools for T1DM. The laboratory production of the reverse-flow immunochromatography kit for GAD65 was done. Furthermore, this kit^s characterization and stability performance has been applied in several ethnics. Importantly, our findings showed that the kit could be stable in long-term utilization for 6-12 months without any decrease in the quality detection against GAD65 protein within the serum samples. Moreover, laboratory testing for several ethnicities improves the kit^s sensitivity for those different samples or races. It is hypothesized that the kit can be further developed for mass production and applied to huge models, particularly for the Indonesian population. Hence, the future exploration of this kit^s potential as a serological biomarker for T1DM could benefit practical and clinical studies. Keywords: GAD65, biomaker, metabolic syndrome, diabetes mellitus Share Link | Plain Format | Corresponding Author (Hendra Susanto)

82	Biophysics	ABS-27
The effect of non-contact electro capacitive cancer therapy on VEGFR2 expression in DMBA-induced rat breast tumor model Endah Sri Palupi (a), Bambang Retnoaji (b), Pudji Astuti (c), Firman Alamsyah (d), Rarastoeti Pratiwi (e*) a) Postgraduate Program, Faculty of Biology, Universitas Gadjah Mada b) Animal Structure and Development Laboratory, Faculty of Biology, Universitas Gadjah Mada c) Department of Physiology, Faculty of Veterinary, Universitas Gadjah Mada d) Center for Medical Physics and Cancer Research, Ctech Labs Edwar Technology, Tangerang e*) Biochemistry Laboratory, Faculty of Biology, Universitas Gadjah Mada * rarastp[at]ugm.ac.id Abstract Angiogenesis contributes to tumor growth and requires signal transduction. VEGFR2 is the primary receptor for the growth factors during tumor angiogenesis. Electro Capacitive Cancer Therapy (ECCT) is an anti-tumor device to inhibit tumor growth. However, its effect on VEGFR2 expression during breast tumor angiogenesis is unclear. Twenty-four rats were divided into 4 groups. Two groups of DMBA-induced rats, while the others were not, with and without ECCT exposure for each. ECCT 150 kHz and 18 Vpp of the AC source were exposed. Mammary glands and breast tumor tissue were collected and preserved. VEGFR2 mRNA expression was measured using qPCR and analyzed using the Livak method with GAPDH normalization. Immunohistochemistry staining was performed on paraffin sections using anti-VEGFR2. The data were statistically analyzed using an independent t-test. The results show that ECCT exposure has no impact on VEGFR2 mRNA expression of normal breast tissue. Insignificant downregulation was expressed in rat breast tumors, in contrast with the ECCT-therapied rat breast tumor. Furthermore, ECCT exposure increased VEGFR2 protein expression significantly, as well as the number of blood vessels. It suggests other mechanisms may be involved in signal transduction during DMBA-induced rat breast tumor angiogenesis. Keywords: angiogenesis- breast tumor- DMBA- ECCT exposure- VEGFR2 Share Link | Plain Format | Corresponding Author (Endah Sri Palupi)

83	Biotechnology	ABS-1
Screening of Padina sp Bioactive Compounds from Coastal Waters of Madura Island as Potential Antioxidants Insafitri, Wahyu Andy Nugraha Marine Science Department, University of Trunojoyo Madura Abstract Padina sp. widely studied in the pharmaceutical and cosmetic fields. Different location will have an influence on the secondary metabolite compounds produced by the algae as a by-product of the adaptation pattern to their environment. Madura Island has the potential for Padina sp. found on the coast of this island but there is no comprehensive data on antioxidant bioactive compounds produced from Padina sp. found on this island. The aims of this study were to identify and analyze antioxidant bioactive compounds contained in Padina sp. from Madura Island. This laboratory research was carried out during July-November 2020 by taking samples of Padina sp. from several locations on the Madura coast and laboratory analysis at the Marine Biology Laboratory, Trunojoyo University Madura. Macroalgae samples obtained will be extracted for proximate levels (carbohydrates, protein, ash, and water), determined for phenolic compounds, and analyzed for their potential as antioxidants using the DPPH method. The results showed that Padina sp. Average water content ranges from 7.54 - 14.52%, average fat content ranges from 0.27 - 1.56%, average protein content ranges from 0.21 - 0.22%, average ash content ranges from 53.78 - 64.22%, fiber content the average ranged from 2.81 - 10.52%, average carbohydrate range 23.05 - 31.66%, Padina sp contained terpenoids, steroids, flavonoids, phenolics, and alkaloids, but did not contain saponins and tannins, phenol levels in Padina sp. averaged between 1.88-71.34%, the average antioxidant level is 25.43 - 44.16%. Keywords: biocative compound, Padina sp, antioxidant, Madura coastal waters Share Link | Plain Format | Corresponding Author (Insafitri Insafitri)

84	Biotechnology	ABS-6
The Use of Bacillus Subtilis as A Healing Agent in Structural Concrete with Recycled Polyethylene Terephthalate Fibres Christian Hadhinata, M. Mirza Abdillah Pratama, and Cynthia Permata Dewi Department of Civil Engineering, Faculty of Engineering, Universitas Negeri Malang, Indonesia Abstract Self-healing concrete is a dependable construction material for cracks recovery via autonomous or autogeneous mechanisms. When bacteria react with water vapour and urea, calcium carbonate (CaCO3) particles are produced, which can repair cracks and protect concrete reinforcement. The addition of fibre to the concrete is expected to reduce crack width and play a role in the bridging mechanism in the calcium carbonate deposit in larger crack. Given the potential of these two materials, the purpose of this research is to examine the mechanical strength of concrete resulting from this bacterial-based concrete innovation, which includes compressive strength and split tensile strength. The study employs experimental methods. The test specimens were concrete with Bacillus subtilis bacteria as a healing agent and fibres extracted from recycled polyethylene terephthalate (PET) fibres, and the reference specimens were the normal concrete. At the age of 28 days, all test specimens moulded in the shape of a standard concrete cylinder of 150x300 mm were tested using Universal Testing Machine. The results showed that using bacteria and 1% of PET reduced compressive and tensile strength by 10% and 1%, respectively, when compared to control specimens. PET has a lower material stiffness than other concrete constituent materials, so its use should be limited to achieve positive results. Keywords: Self-healing concrete, Bacillus subtilis bacteria, Cracks recovery, Recycled polyethylene terephthalate, Fibres Share Link | Plain Format | Corresponding Author (M. Mirza Abdillah Pratama)

85	Biotechnology	ABS-12
Ploidy Level of Endangered-Endemic Orchid Vanda Sumatrana Schltr Regenerated Plantlets Dyah Retno Wulandari 1, a), Tri Muji Ermayanti 1,b), Erwin Al Hafiizh 1,c), Dwi Murti Puspitaningtyas 2,d), Suluh Normasiwi 3,e), and Syarifin Firdaus 4,f) 1(Research Center for Genetic Engineering-National Research and Innovation Agency: National Integrated Center for Genomic, Tropical Biodiversity and Environment Jl. Raya Jakarta-Bogor No.Km. 46, Cibinong, Kec. Cibinong, Kabupaten Bogor, Jawa Barat 16911). 2(Research Center for Plant Conservation, Botanic Gardens, and Forestry: Jl. Ir. H. Juanda No.13, Paledang, Kecamatan Bogor Tengah, Kota Bogor, Jawa Barat 16122) 3(Research Center for Plant Conservation, Botanic Gardens, and Forestry:Jl. Kebun Raya Cibodas, Sindangjaya, Kec. Cipanas, Kabupaten Cianjur, Jawa Barat 43253) 4(Orchid Bloem and Farm-Malang East Java Indonesia: Jl. Satsuitubun, Greenliving Residence D37 Malang, 65149). Abstract Vanda Sumatrana is an endangered-endemic Indonesian orchid according to Ministry of Environment and Forestry, Indonesia, Regulation No.106 of 2018. Plant tissue culture is a suitable technique for in vitro propagation and conservation. The ploidy level of plantlet needs to be investigated to observe genetic diversity or stability. This study aimed to analyze the ploidy level of 10-week-old regenerated plantlets of V. sumatrana compared to their mother stock plant that has been stored on in vitro culture for 3 years. Observations were conducted on 70 regenerated-plantlets from 24 parent plants. Analysis of the ploidy level was carried out using a flow cytometer. The results showed that 70.83% parental shoots or were diploids and 29.16% were tetraploids, 72.46 % regenerated shoots were diploids and 27.54% were tetraploids. Mean value of PI ranged from 107.472 to 205.162 for diploid shoots, and ranged from 258.525 to 399.255 for tetraploid shoots. This indicated endopolyploidy occurred between regenerants and their parent plants after a long period of cultures. Our finding also showed endoreduplication phenomena in this orchid species, indicated by multiple peaks of flow cytometry histograms. Keywords: flow cytometry- endopolyploidy- endoreduplication- Vanda sumatrana- plantlet Share Link | Plain Format | Corresponding Author (Dyah Retno Wulandari)

86	Biotechnology	ABS-15
Characterisation of fresh concrete incorporated with Bacillus subtilis and recycled Polyethylene Terephthalate M. Mirza Abdillah Pratama, Indriyani Indriyani, Cynthia Permata Dewi, and Christian Hadhinata Department of Civil Engineering, Faculty of Engineering, Universitas Negeri Malang, Indonesia Abstract The workability and characteristics of concrete when it is fresh are factors that influence its strength and durability as a building structural element. The goal of this research was to compare the physical properties of fresh concrete based on Bacillus subtilis bacteria and Polyethylene Terephthalate (PET) fibre to those of normal concrete. Laboratory tests include slump collapse, volume weight, fresh concrete temperature, and compaction factor testing were conducted. In addition, the concrete was subjected to a permeability test to determine its ability to pass water. The test results show that the slump value, volume weight, and compaction factor of the innovation concrete are lower than those of normal concrete. This is due to the use of synthetic fibres, which cause the concrete mixture to clump and become more difficult to work with. The temperature of the mixture showed no significant difference. Concrete characteristics are improved in terms of permeability, where innovative concrete produces better impermeability to dispel the rate of water vapour entering the internal concrete, preventing the concrete reinforcement from rusting. Keywords: self-healing concrete, fresh concrete, workability, slump collapse, volume weight, temperature, compaction factor Share Link | Plain Format | Corresponding Author (M. Mirza Abdillah Pratama)

87	Biotechnology	ABS-20
Enhancing Brackish Water Tilapia Seed Production with Biofloc Technology Abidin Nur (1), Indrian Rizka Amalia (1), Dian Permana (1), Ratna Indria Sari (1), Endah Soetanti (1), Siswanto (2) (1) Research Center for Fishery, National Research and Innovation Agency, Indonesia (2) Main Center for Brackish Water Aquaculture Jepara, Ministry of Marine Affairs and Fisheries, Indonesia Abstract Preliminary study on biofloc technology for brackish water tilapia seed production were evaluated by using two different stocking densities of 3.000 pcs.m-3 and 6.000 pcs.m-3. Fish larviculture was performed on concrete tanks measured on 5.5 x 1.8 x 0.8 m (active volume 5 m-3) located in semi-indoor building. Biofloc formation were stimulated by adding molasses as carbon source by adjusting C : N ratio to around 10 or more. The water salinity increased gradually after 8th day and reached up to 15 ppt during harvest. The result showed that biofloc volume was recorded at a range of 2-10 mL.L- with TAN (Total ammonia nitrogen) concentration of less than 0.5 ppm, indicating that the system had worked properly. Seed survival rate was 77-79 % and biomass was 0.60 - 0.85 kg.m-3, respectively. It can be concluded that biofloc technology is an alternative to enhance seed production of brackish water tilapia, and is expected to increase the growth of tilapia in the pond. Seed density and biomass need to be studied further regarding tilapia^s growth in pond. Keywords: biofloc technology, biomass, brackish water tilapia seed, survival rate Share Link | Plain Format | Corresponding Author (Abidin Nur II)

88	Biotechnology	ABS-22
Botanical Cultivation And Ethno-pharmacological Perspectives Of Gynura pseudochina : An Anthocyanin-rich Medicinal Plants Aiziah Ayu Pratami (a), Nurul Jadid (a*) a) Department of Biology, Faculty of Science and Data Analytics, Institut Teknologi Sepuluh Nopember, Sukolilo, Surabaya, Indonesia 60111 *nuruljadid[at]bio.its.ac.id Abstract Indonesia is a tropical country that has a high diversity of flora in the world, including plants that have pharmaceutical roles. Gynura pseudochina (L.) DC) or locally named as Daun Dewa, is a medicinal plant that contains bioactive compounds. Various scientific evidences proved that this plant has been long term used by societies worldwide. The pharmacological activities of this plant include antioxidants, anti-diabetes, anticancer, anti-obesity, antimicrobial etc. Therefore, G. pseudochina represents a high economic value of medicinal plants. Consequently, their market demand is getting increased. Tissue culture method is considered as an alternative method to accelerate high quality of plant cultivation. In addition, it is also used to increase the production of secondary metabolites. Several attempts of plant tissue culture methods have been made to boost the production of plant secondary metabolites. It includes the use of biotic and abiotic elicitors. This review focuses on botanical and ethno-pharmacological perspectives of G. pseudochina. Moreover, the cultivation aspects will be only emphasized on in vitro culture of Daun Dewa. Thus, the information provided in this review will serve as references for future research in plant improvement and large-scale production of important plant secondary metabolites. Keywords: Cultivation- Ethno-pharmacology- Gynura pseudochina- Plant secondary metabolites- Plant tissue culture Share Link | Plain Format | Corresponding Author (Nurul Jadid)

89	Biotechnology	ABS-23
Effect of LED Light and Different Plant Growth Regulators (PGRs) on Organogenesis of Weevil-wort (Curculigo latifolia) In Vitro May Fiatus Sholihah (a), Amalia Salsabila Rifani (a), Wirdhatul Muslihatin (a), Nurul Jadid (a*) a) Department of Biology, Faculty of Science and Data Analytics, Institut Teknologi Sepuluh Nopember, Surabaya 60111, Indonesia *nuruljadid[at]bio.its.ac.id Abstract One of the obstacles in the cultivation of weevil-wort (Curculigo latifolia) are its seed recalcitrance and high variability among its accessions. Plant tissue culture serves as an alternative technology to provide high quality of C. latifolia seeds. This study aims to determine the effect of LED lighting and plant growth regulators (PGRs) on the organogenesis of weevil-wort (C. latifolia) in vitro. Seeds of C. latifolia were planted in the Murashige & Skoog (MS0) media- MS supplemented with IBA (1 mgL-1) and BAP (2 mgL-1)- and BAP alone (2 mgL-1). All culture media were then treated with different type of LED light: red, blue, and white light. Our results showed 100% of seeds placed on the MS0 media were able to germinate in all type LED light treatment. About 67% of seeds, grown in the MS supplemented with IBA+BAP and treated with blue light were also able to develop shoots. Interestingly, we noticed that treatment on MS with BAP alone and LED red light gave no shoot-regenerated seed. Best performance on shoot height was obtained in the MS0 media treated with all LED light, which range from 1.5 - 1.87 cm. Meanwile, the lowest shoot height of C. latifolia was obtained when the seeds were placed on MS with BAP alone (2 mgL-1). Keywords: Curculigo latifolia- LED light treatment- Plant growth regulators- Plant tissue culture Share Link | Plain Format | Corresponding Author (Nurul Jadid)

90	Biotechnology	ABS-24
Patchouli (Pogostemon cablin Benth.): Progress on its In Vitro Cultivation and Pharmacological Perspectives Anisa Esti Rahayu (a), Nurul Jadid (a*) a) Department of Biology, Faculty of Science and Data Analytics, Institut Teknologi Sepuluh Nopember Surabaya, 60111, Indonesia *nuruljadid[at]bio.its.ac.id Abstract Patchouli (Pogostemon cablin Benth.), belonging to the family Lamiaceae, is a medicinal plant native to the Philippines and grows wild in many South Asian countries, including Indonesia. It is widely used in the pharmaceutical industry because it contains various bioactive compounds. These bioactive compounds include flavonoids, terpenoids, phytosterols, organic acids, lignins, glycosides, alcohols, pyrones, and aldehydes. The main bioactive compounds are patchouli alcohol, patchoulene epoxide, &#945--patchoulene, &#946--patchoulene, &#945--bulnesene, seychellene, norpatchoulenol, pogostone, eugenol, pogostol, and pachypodol. The pharmacological effects of these compounds include antioxidant, antimicrobial, antitumor, antidiabetic, anti-inflammatory, anti-oxidative, anti-peptic ulcer, anti-hypertensive, antiplatelet, antidepressant, antithrombotic, antimutagenic, antiemetic, effect on ischemia, immunoregulatory, analgesic, aphrodisiac, fibrinolytic, and cytotoxic activities. So, this medicinal plant is a leading commodity in the global market and has a high economic value. In vitro culture techniques have been widely used for the fast production of uniform, pathogen-free, and high-quality plants on a commercial scale. This review summarizes the recent progress in the plant in vitro cultivation and pharmacological aspects of patchouli and provides a reference for future studies focused on the mass production of patchouli plants. Keywords: Bioactive compounds- In vitro culture- Pharmacology- Pogostemon cablin Benth Share Link | Plain Format | Corresponding Author (Nurul Jadid)

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