In-vitro toxicity study of Chitosan/Alginate nano-encapsulated single garlic extract on 3T3-L1 cell Sri Rahayu Lestari (1*), Hartatiek (2), Abdul Gofur (1), Yuslinda Annisa (1), Nik Ahmad Nizam Nik Malek (3,4)
(1) Department of Biology, Faculty Mathematics and Natural Science, Universitas Negeri Malang, Malang, East Java, Indonesia
*srirahayulestari[at]um.ac.id
(2) Department of Physics, Faculty Mathematics and Natural Science, Universitas Negeri Malang, Malang, East Java, Indonesia
(3) Department of Biosciences, Faculty of Science, Universiti Teknologi Malaysia, 81310 Johor Bahru, Malaysia
(4) Center for Sustainable Nanomaterials, Ibnu Sina Institute for Scientific and Industrial Research, Universiti Teknologi Malaysia, 81310 Johor Bahru, Malaysia
Abstract
Single garlic (Allium sativum) is one of the local plants that is used as a medicinal ingredient. Single garlic^s active compounds such as Allicin are easily degraded in the digestive tract, so the expected health effects are not optimal. The encapsulation process can increase the solubility and bioavailability of active compounds in single garlic. Chitosan and alginate are natural biopolymers that can be used as nanoparticle candidates for encapsulation of single garlic active compounds because they are not toxic. This study aims to formulate and test the cytotoxicity of chitosan/alginate nano encapsulated single garlic extract (CAN-SGE) in vitro. The methods used include single garlic extraction with ethanol solvent, CAN-SGE formulation and characterization, and CAN-SGE toxicity test. Characterization of CAN-SGE using Particle Size Analyzer (PSA). A cytotoxicity test was carried out using the MTT assay method. The results showed that the mean droplet size of CAN-SGE was 603,5 nm, the polydispersity index (PDI) was 0,547 and the Zeta potential was -15,7 mV. CAN-SGE has low toxicity at concentrations below 1000 ppm with a cell viability value of 88%. Chitosan/alginate nano encapsulated has the potential to be a drug delivery system for single garlic extract.
Keywords: Single Garlic- Chitosan- Alginate- Nanoencapsulation- Toxicity
