Morphological Characterization and Antioxidant Activity Test of Single Garlic Extract-loaded Chitosan-Alginate Nanoencapsulation Ikfi Nihayatul Mufidah (a), Sri Rahayu Lestari (a*), Siti Imroatul Maslikah (a), Sunaryono (b)
a) Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, Jl. Semarang 5, Malang 65145, Indonesia
*srirahayulestari[at]um.ac.id
b) Department of Physics, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, Jl. Semarang 5, Malang 65145, Indonesia
Abstract
Single garlic is often used in traditional medicine. The compounds include organosulfur and phenolic which have antioxidant activity. Allicin is a major component of antioxidant compounds, but it is unstable and easily degraded in the digestive. Chitosan-alginate nanoencapsulation promises to protect allicin from being degraded, so its antioxidant activity is more optimal in the body. The purpose of this study was to characterize the morphology of single garlic extract-loaded chitosan-alginate nanoencapsulation (SGE-CANe) and to examine the effect of chitosan-alginate nanoencapsulation (CANe) on the antioxidant activity of single garlic. The research procedure includes single garlic extraction, preparation of SGE-CANe, morphological of SGE-CANe, and antioxidant activity test. The morphological characterization of SGE-CANe was using SEM, while the antioxidant activity was using the DPPH test. The results showed that the morphology of SGE-CANe was spherical with a size of < 500 nm. The results of the antioxidant activity test showed that the CANe had an effect on the antioxidant activity of single garlic. The antioxidant activity of SGE-CANe at 24 000 ppm was 65%, while the antioxidant activity of SGE was 52%. CANe protects antioxidant compounds of single garlic extract so that antioxidant activity is more optimal.
Keywords: Single garlic- Allicin- Chitosan-alginate nanoencapsulation- Morphology, Antioxidant activity
