Effect of LED Light and Different Plant Growth Regulators (PGRs) on Organogenesis of Weevil-wort (Curculigo latifolia) In Vitro May Fiatus Sholihah (a), Amalia Salsabila Rifani (a), Wirdhatul Muslihatin (a), Nurul Jadid (a*)
a) Department of Biology, Faculty of Science and Data Analytics, Institut Teknologi Sepuluh Nopember, Surabaya 60111, Indonesia
*nuruljadid[at]bio.its.ac.id
Abstract
One of the obstacles in the cultivation of weevil-wort (Curculigo latifolia) are its seed recalcitrance and high variability among its accessions. Plant tissue culture serves as an alternative technology to provide high quality of C. latifolia seeds. This study aims to determine the effect of LED lighting and plant growth regulators (PGRs) on the organogenesis of weevil-wort (C. latifolia) in vitro. Seeds of C. latifolia were planted in the Murashige & Skoog (MS0) media- MS supplemented with IBA (1 mgL-1) and BAP (2 mgL-1)- and BAP alone (2 mgL-1). All culture media were then treated with different type of LED light: red, blue, and white light. Our results showed 100% of seeds placed on the MS0 media were able to germinate in all type LED light treatment. About 67% of seeds, grown in the MS supplemented with IBA+BAP and treated with blue light were also able to develop shoots. Interestingly, we noticed that treatment on MS with BAP alone and LED red light gave no shoot-regenerated seed. Best performance on shoot height was obtained in the MS0 media treated with all LED light, which range from 1.5 - 1.87 cm. Meanwile, the lowest shoot height of C. latifolia was obtained when the seeds were placed on MS with BAP alone (2 mgL-1).
