Characterization and Hemocompatibility Assay of In-Vitro Single Garlic Extract-loaded Chitosan-Alginate Nanoencapsulation
Amalia Nur Rahma (a), Sri Rahayu Lestari (a*), Abdul Gofur (a), Siti Imroatul Maslikah (a)

a) Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, Jl. Semarang 5, Malang 65145, Indonesia
*srirahayulestari[at]um.ac.id

Abstract

Single garlic is a tropical plant with high bioactive compounds. Most bioactive compounds were found in single garlic is allicin but has low bioavailability. Allicin was classified to Reactive Sulfur Species (RSS) and has the ability to damage eukaryotic cells, for example is red blood cells. The damaged of red blood cells was minimized with a specific mechanism using the Drug Delivery System (DDS) with Chitosan-Alginate Nanoencapsulation (CANe). The aim of this study is to characterization and hemocompatibility assay of red blood cells. Single garlic extraction method using maceration with ethanol 70%. Optimal formulation of SGE-CANe determined using characterization with Particle Size Analyzer (PSA) and hemocompatibility assay. PSA of SGE-CANe such as Z-Average (390,540 nm), Polydispersity Index (PDI) (0,609), and zeta potential (-23,067 mV) shows that SGE-CANe categorized into optimal DDS. Hemocompatibility assay shows that SGE-CANe has low hemolysis percentage than SGE. The result of hemolysis percentage SGE-CANe does not cause the damage of red blood cells. Thus, it can be concluded that SGE-CANe was optimal increasing bioavailability of allicin compounds, hence SGE-CANe was compatible with red blood cells.

Keywords: SGE-CANe- Hemocompatibility- Single garlic

Topic: Bionanotechnology