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31	Bioinformatics	ABS-80
The Effectiveness of Educational Media with Role Play towards Improving Mother Skills as an Effort to Prevent Sexual Violence in Pre-School Age Children Nurul Laili, Erna Handayani, Ro^isah STIKES HAFSHAWATY PESANTREN ZAINUL HASAN PROBOLINGGO Abstract Abstract. Sexual violence against children is a form of sexual deviation that is very life-threatening, especially having a destructive impact both physically and psychologically. Meanwhile, in Probolinggo Regency, there are 35 cases of sexual violence against children. The essential purpose of this study is to analyze the effectiveness of providing education using the role-play method for mothers^ skills in preventing sexual violence with pre-school-age children. The design of this study is quasi-experimental. The sampling used purposive sampling with a total sample of 76 respondents divided into 38 respondents as an intervention group and 38 respondents as a control group. Data was collected using a questionnaire of respondents^ characteristics and an observation sheet ^Child sexual abuse prevention practices.^ Data analysis used the Wilcoxon test. The results showed the effectiveness of education with the role-play method on maternal skills to prevent sexual violence against children with a P-value of 0.005. Educational media with the role-play method can be applied as an effort to improve maternal skills as an effort to prevent sexual violence in pre-school-age children. Families, nursing professions, educational institutions, and subsequent research are expected to participate in efforts to prevent sexual violence in children. Keywords: educational media, role play, mother skills, sexual violence Share Link | Plain Format | Corresponding Author (Nurul Laili)

32	Bioinformatics	ABS-84
Study Investigation The Risk Factors of Toddlers With Low Nutritional Status in The North Coast of Java, Indonesia 1) Yulia Rachmawati Hasanah, 2) Widya Addiarto, 3) Mega Silvian Natalia 1, 2) Department of Nursing, STIKes Hafshawaty Pesantren Zainul Hasan, Probolinggo, Indonesia 3) Department of Midwifery, STIKes Hafshawaty Pesantren Zainul Hasan, Probolinggo, Indonesia Abstract Abstract. Today, one of the problems in the world that is never finished was low nutritional status. Toddlers nutritional are the measure of the success nutritional status. Nutritional status as a form for assessment the toddlers development that is used to determine the nutritional intake needed. Each toddlers has a different nutritional status, depending on gender, age, weight, height, and head circumference by checking directly through the posyandu or pediatrician. The risk of low nutritional status in children is caused by several factors, including internal factors and other factors. external. UNICEF in 2020 estimates that 45.4 million children under five years of age are acutely malnutrition. In 2021 the incidence of low nutrition status with stunting in Indonesia is 24.4%, East Java Province is 23.5% and Probolinggo Regency is 23.3%. This study is a descriptive study with a cross-sectional approach, with a population of all mothers who have stunting toddlers. The sampling method is total sampling and the variables including a history of birth weight, history of exclusive breastfeeding, parenting nutrition, maternal age at delivery, maternal age at marriage, mother^s education, mother^s occupation, family income, access and quality of health services, number of Antenatal Care visits, clean water facilities, sanitation facilities. This study uses a questionnaire distributed to all respondents via google form then the data is processed to determine the dominant factors that influence the incidence of stunting in children. Keywords: The Risk Factors, Low Nutritional Status Share Link | Plain Format | Corresponding Author (Yulia Rachmawati Hasanah)

33	Bioinformatics	ABS-88
Potential of Clinacanthus nutans in the treatment of diabetes mellitus and molecular mechanisms prediction based on network pharmacology Nurlaili Susanti(1,2*), Arifa Mustika(3), Junaidi Khotib(4) (1) Doctoral Program of Medical Science, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia (2) Faculty of Medicine and Health Science, UIN Maulana Malik Ibrahim, Malang, Indonesia (3) Department of Anatomy, Histology, and Pharmacology, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia (4) Department of Pharmacy Practice, Faculty of Pharmacy, Universitas Airlangga, Surabaya, Indonesia. *)nurlaili.susanti-2020[at]fk.unair.ac.id Abstract Clinacanthus nutans is rich in phytoconstituents and is considered a promising medicinal plant. This study aims to review the potential of C. nutans as an anti-diabetic and to explore its molecular mechanism based on network pharmacology. A literature review was conducted on articles, obtained from Scopus and Google Scholar databases, showed that C. nutans has an anti-diabetic effect. For network pharmacology analysis, the bioactive compounds of C. nutans was acquired from previous related research and SwissADME was used to predict its Drug-likeness based on Lipinski rule and oral bioavailability score. The overlapping between diabetes-associated genes, retrieved by DisGeNET database, and the predicted C. nutans target genes were visualized by Venn Diagram. Protein-protein interaction networks of these overlapping genes were identified using String-db. Those interactions construct a PPI network which is then assessed by Cytoscape 3.9.1 software to analyze potential genes and then used to construct a compound-target network. Finally, the signaling pathways in which these genes are involved were analyzed based on the KEGG database. These results imply that C. nutans has potential as anti-diabetic through several signaling pathways associated with diabetes mellitus. This provides a scientific basis for further research on the anti-diabetic mechanism of C. nutans. Keywords: Clinacanthus nutans, diabetes, medicine, network pharmacology Share Link | Plain Format | Corresponding Author (Nurlaili Susanti)

34	Bioinformatics	ABS-89
Effectiveness of Utilizing Local Production of Moringa Oleifera Lamk with Sauropus Androgynus as an Effort to Increase Breast Milk Production Mariani1,a), Rizka Yunita1,b), Iin Aini Isnawati1,c) Department of Nursing, Sekolah Tinggi Ilmu Kesehatan Hafshawaty Pesantren Zainul Hasan, Probolinggo, Indonesia Abstract The presence of a baby is the happiest moment and is eagerly awaited by parents. Exclusive breastfeeding is the duty of a mother after giving birth, and the happiest thing for a mother is when she is able to give breast milk to her baby. But in reality there are still many mothers who experience problems in giving breast milk because milk production does not come out in the first days of breastfeeding, thus making mothers feel anxious and worried because they cannot meet the nutritional needs of their babies and finally give formula milk. The purpose of this study was to determine the effectiveness of Moringa oleifera Lamk (Leaves Kelor) and Sauropus Androgynus (Leaves Katuk) on increasing breast milk production. The method used in sampling is using the total sampling method. The research sample was all pregnant women 37-40 weeks of gestation who gave birth normally at the Independent Midwife Practice. This type of research is a quasi-experimental research design with two group pre-posttest design. The results showed that giving and Sauropus Androgynus (Leaves Katuk) was more effective than Moringa oleifera Lamk (Leaves Kelor) in increasing breast milk production with a p value of 0.05. The conclusion is that Moringa oleifera Lamk (Leaves Kelor) and Sauropus Androgynus (Leaves Katuk) can be given and consumed for third trimester pregnant women and Moringa oleifera Lamk (Leaves Kelor) is more effective in increasing breast milk production. Keywords: Moringa Oleifera Lamk, Sauropus Androgynus, Breast Milk Production Share Link | Plain Format | Corresponding Author (Mariani Mariani)

35	Bioinformatics	ABS-90
Antidiabetic Effects of Spilanthes acemlla (Wall. ex DC.) C.B. Clarke ex Hook. flowers in silico studies Muhammad Shofi*, Rifqi Hilman, and Daradjatul Aulia Departement of Biology, Faculty of Mathematics and Natural Science, Malang Satte University, Malang 65145, East Java, Indonesia Abstract Context: One therapeutic approach to lowering blood sugar levels posprandial is to slow down or inhibit the absorption of glucose through inhibition of the carbohydrates hidrolitic enzyme such as &#945--amylase and &#945--glucosidase. In silico study can efficiently reduce the time and cost of such screening antidiabetic agent candidates. Aims: To elucidate the molecular mechanisms underlying Antidiabetic Effects of Spilanthes acemlla (Wall. ex DC.) C.B. Clarke ex Hook. flowers in silico analysis. Methods: Eight bioactive compounds of Spilanthes acemlla (Wall. ex DC.) C.B. Clarke ex Hook (spilanthol, amyrinester, stigmasterol, miricilic alcohol glycosides, sitosterol, saponins, acmelonate, and triterpenes were used for this study. The canonical smiles was investigated bioactive compound were retrieved from PubChem and Insert into (PASS) server to determine the biological activity using. Compounds were docked with several protein targets, including caspase 3, Bax and Bcl-2. Molecular docking was done by Pyrx 0.8 software and visualized by Discovery Studio software. The pharmacological properties of investigated bioactive compounds were analyzed using the SwissADME web server. Toxicity properties were anlyzed using Protox. Results: The eight bioactive compounds of Spilanthes acemlla. flowers have Antidiabetic Effects properties. Results showed that various biological activities have a potential as Antidiabetic Effects of all investigated compounds including hypoglycemic effects. Based on SwissADME analysis known that most of the all investigated bioactive compounds showed optimum lipophilicity, size, polarity, solubility, and flexibility. candidate, so it needs to be studied further. Based on Protox analysis known that most of the investigated bioactive compounds showed in active hepatotoxicity, cytotoxicity and mutagen Conclusions: The bioactive compounds of Spilanthes acemlla flowers might potentially modulate hypoglycemic effects Keywords: Spilanthes acemlla, In silico, Antidiabetic effect Share Link | Plain Format | Corresponding Author (Muhammad Shofi Amrilah -)

36	Bioinformatics	ABS-91
Computational Bioactivity, Pharmacological, Biofarmasetic And Toxicity Properties Analysis For Anti-Breast Cancer Acmella olerac(L.) R.K.Jansen Flowers In Silico Studies Muhammad Shofi* and Rifqi Hilman, Departement of Biology, Faculty of Mathematics and Natural Science, Malang Satte University, Malang 65145, East Java, Indonesia Abstract The development of apoptotic agents from natural plant products may have a beneficial effect as a promising candidate for cancer therapy. The study about the efficacy of Acmella oleraceae (L.) R.K.Jansen Flowers on breast cancer are still limited. Aims: To elucidate the molecular mechanisms underlying the anti-breast cancer activity of Acmella oleraceae by in silico analysis. Methods: seventeen bioactive compounds of Acmella olerac(L.) {(N-Alkylamide = spilanthol), Alpha dan beta amyrin ester, stigmasterol, Beta sitosterol, Alpha 1-sitosterol, 3-acetyl aleuritic acid, scopoletin, vanillic acid, trans ferulic, trans isoferulic), (acmelonate = 7Z,9E)-2-oxo-undeca-7,9-dienyl 3-methylbut-2-enoat), and beta - caryophyllene, Beta- pinene, myrcene, caryophyllene oxide, limone)} were used for this study. The canonical smiles were investigated and bioactive compounds were retrieved from PubChem and Insert into PASS server to determine the biological activity using. Compounds were docked with several protein targets, including ESR1, MAP2K2 and PGR. Molecular docking was done by Pyrx 0.8 software and visualized by Pymol and Discovery Studio software. The pharmacological properties of investigated bioactive compounds were analyzed using the SwissADME web server. Toxicity properties were analyzed using Protox. Results: The seventeen bioactive compounds of Acmella olerac(L.) R.K.Jansen. Flowers have anticancer properties and might be expected to be involved in estrogen pathways related to breast cancer. Results showed that various biological activities have a potential as anticancer of all investigated compounds including TP53 expression enhancer,Apopotosis agonist, Ovulation inhibitor, Antineoplastic, Steroid synthesis inhibitor,Caspase 3 stimulant, Caspase 8. Based on SwissADME analysis, it is known that most of the all investigated bioactive compounds show optimum lipophilicity, size, polarity, solubility, and flexibility. candidate, so it needs to be studied further. Keywords: Acmella oleraceae- Anti-cancer- breast cancer- bioactive compounds Share Link | Plain Format | Corresponding Author (Muhammad Shofi Amrilah -)

37	Bioinformatics	ABS-111
Anti-Inflammatory Potential of Cat^s Whiskers Leaf (Orthosiphon stamineus) through inhibition of NF-kB and INOS proteins: in silico study. Rizqi Zidni Hidayati1, Masruri2, and Widodo1* 1Biology Departement, Faculty of Mathematic and Natural Science, Universitas Brawijaya Malang, Indonesia. 2Chemistry Departement, Faculty of Mathematic and Natural Science, Universitas Brawijaya Malang, Indonesia. *)Corresponding author: widodo[at]ub.ac.id Abstract ABSTRACT. Inflammation is a physiological response in humans to foreign organisms, including pathogens, dust particles, bacteria, and viruses. An exaggerated inflammatory response leads to pathological conditions, including atherosclerosis, autoimmune disorders, neurodegenerative diseases, and cancer. Extract leaves O.stamineus to have the potential to be anti-inflammatory. This study aimed to determine the potential of O.stamineus as an inhibitory anti-inflammatory through NF-kB and INOS proteins with an in silico approach. Screening of phytochemical compounds using the LCHRMS tool, molecular docking was carried out using the Discovery Studio 2019 and PyRx 8.0 software, and molecular dynamics were carried out using the WebGRO website. The results of the phytochemical screening contained 28 compounds contained in the leaves of O.stamineus. NF-kB protein binds to trifolin (-6.6 kcal/mol) and ursolic acid (-7.2 kcal/mol) ligands. The iNOS protein binds with pinostrobin (-9.2 kcal/mol) and pinocembrin (-9.2 kcal/mol) ligands. NF-kB and INOS proteins have stable molecular dynamic activity. This study concludes that several compounds contained in the leaf of O.stamineus can inhibit NF-kB and INOS proteins so that they have the potential to be anti-inflammatory. Keywords: Antiinflammation, Orthosiphon stamineus, NF-kb, INOS. Share Link | Plain Format | Corresponding Author (Rizqi zidni Hidayati)

38	Bioinformatics	ABS-116
In Silico Analysis of Irisflorentin and Irilone Compound on Stone Root Tubers ( Gerrardanthus macrorhizu Harv.ex Benth. & Benth. &HooK.f.) as an Antitussive Maria Angelina Genere Koban (a*) Sri Rahayu Lestari (b), Frida Kunti Setiowati (c) Department of Biology Postgraduate State University of Malang. Jln. Semarang No.5 Abstract Cough as a defensive reflex can protect the respiratory tract from the entry of foreign objects. Dextromethorphan as an antitussive drug is antagonistic to NMDA receptors. This study aims to analyze the potential of irisflorentin and irilone as antitussives based on their binding affinity to the NMDA receptor, biooral potential, and toxicity by in silico approach. The results showed that dextromethorphan and irilone bind to NMDA receptors on amino acid residues, namely ILE A: 85, MET A: 145, ALA B: 888, SER B: 885, THR B: 884 while dextromethorphan and irisflorentin bind to NMDA receptors at residues. The same amino acids are GLU A: 84, ILE A: 85, MET A: 145, SER A: 81, THR B: 884, and SER B: 885. The affinity value of irisflorentin (-6.5 kcal/mol) is smaller than that of dextromethorphan (-6.4 kcal/mol) and irilone -6.4 kcal/mol is the same as dextromethorphan. This shows that both compounds have almost the same ability to bind to the NMDA glutamate receptor, so it is predicted that both have similar potential to dextromethorphan as an antitussive. The results of the toxicity test showed that both could be taken orally and had little risk if consumed not exceeding the exposure threshold. Keywords: Antitussive, Dextromethorphan, Stone Root Tubers, In Silico Share Link | Plain Format | Corresponding Author (Maria Angelina Genere Koban)

39	Bioinformatics	ABS-134
Insilico Studies of Illicium Verum on Angiotensin-Converting Enzyme and Angiotensin II Receptor as Antihypertensive Agents Andri Tilaqza*, Zaenab Aqilah Pharmacy Department, Medical Faculty, University of Islam Malang, Malang, Indonesia *Corresponding author: andri.tilaqza[at]unisma.ac.id Abstract Illicium verum is one of the plants used empirically for hypertension and diabetes. It contains approximately 73 active compounds. This study aims to find out the candidates of active compounds that have the potential as antihypertensives. Active compounds that meet the Lipinski criteria were simulated against Angiotensin-Converting Enzymes and Angiotensin II Receptors using Captopril and Valsartan as reference drugs. The research method was carried out using computational simulations of molecular docking, then visualized using Biovia Discovery Studio Visualizer and pharmacokinetics prediction analysis through the pkcsm site. The results obtained 49 of the 73 compounds meeting the Lipinski criteria. There was 37 compounds having free energy value greater than captopril (-5.9 kCal/mol), including Catechins (-8.4 kCal/mol), Allo aromadendrene (-7.9 kCal/mol) and Longifolene (-7.8 kCal/mol). None of the compounds has free energy value greater than valsartan (-8.5 kCal/mol), including Delta cadinene (-7.9 kCal/mol), Alpha muurolene (-7.9 kCal/mol), Caryophyllene (-7.8 kCal/mol). Based on the similarity of chemical interactions, Alpha santalene, D-fenchone, Fenchone had similarities with Captopril, while Alpha Copaene, Santalene, Feniculin had similarities with Valsartan. Almost all compounds had a fairly good pharmacokinetic profile. Illicium verum has potential as an antihypertensive by molecular docking. Keywords: hypertension, illicium verum, molecular docking, pharmacokinetic Share Link | Plain Format | Corresponding Author (andri tilaqza)

40	Bioinformatics	ABS-136
5-O-acetylpinostrobin Analogs as Breast Cancer Drugs: Docking and Linear Regression QSPR for ER Alpha Inhibitors Anita Puspa Widiyana(a), Tri Widiandani(b), Siswandono Siswodihardjo(b*) a)Doctoral Program of Pharmaceutical Sciences, Faculty of Pharmacy, Universitas Airlangga, Surabaya, Indonesia a)Department of Pharmacy, Faculty of Medicine, Universitas Islam Malang, Malang, Indonesia b) Department of Pharmaceutical Sciences, Faculty of Pharmacy, Universitas Airlangga, Surabaya, Indonesia *prof.sis[at]ff.unair.ac.id Abstract Breast cancer cases in 2020 have ranked first in the world and second in Indonesia, which are generally caused by 75 percent ER alpha overexpression. Long-term use of Tamoxifen as a breast cancer drug has been shown to result in drug resistance. Therefore, it is necessary to develop cancer drugs that have better activity. This study aimed to determine the prediction of anti-breast cancer activity and the QSPR relationship of 5-O-acetylpinostrobin analogs. Prediction of anti-breast cancer activity was used by the AutoDock Tools with the type of estrogen receptor alpha (PDB: 6V87). Parameters that have been selected include free energy binding and inhibition constant. Physicochemical properties (log P, HBA, MR, and TPSA) and excretion parameters (Cltot) were determined via pkCSM online. The relationship between physicochemical properties and total clearance through the QSPR equation was determined by SPSS. The results showed that 5-O-(dimethylamino)benzoylpinostrobin has the highest affinity for ER inhibitors, as seen from the value of free energy binding of -7.86 kcal/mol and Ki of 1.73 micromolar. The best QSPR equation was Log (1/Cltot)=0.130RB-0.034MR+0.366LogP+1.534 (n=14, r=0.749, R2=0.561, F=6.825, Sig.=0.004). The physicochemical parameters that are most influenced by the QSPR equation include rotatable bond, molar refractivity, and Log P. The outcome of this research is that 5-O-(dimethylamino)benzoylpinostrobin has the potential to be developed into a breast cancer drug through ER alpha inhibitors. Keywords: 5-O-acetylpinostrobin, breast cancer, ER alpha, molecular docking, QSPR Share Link | Plain Format | Corresponding Author (Anita Puspa Widiyana)

41	Bioinformatics	ABS-144
Diabetes Self Management Education Increasing Self-Care For Type II Diabetes Mellitus Patients Dodik Hartono1),Titik Suhartini2),Yulia Rachmawati Hasanah3) STIKES Hafshawaty Pesantren Zainul Hasan Probolinggo, Indonesia Abstract Type II Diabetes Mellitus is a glucose disorder caused by impaired insulin secretion which can cause various chronic complications. (DSME) can improve skills in performing self-care to prevent diabetic ulcers. This study aims to determine the effect (DSME) on self-care behavior at the Holistic Nursing Therapy Clinic in Probolinggo. This type of research is a pre-experimental research with a one group pre-post design. The population of 35 respondents and a sample of 32 respondents who met the research inclusion requirements were selected through accidental sampling technique. The instrument used was a Summary of Self-care Behavior Assessment Sheet (SSDCA), then data were collected through the Editing, Coding, Scoring, and Tabulating processes. The data obtained were analyzed using the Wilcoxon signed rank test. The results of the study before being given (DSME) showed that the most self-care behavior results were those classified as less, namely 21 (65.6%) respondents. After (DSME) most of the self-care behaviors were given enough, namely 12 (37.5%) respondents. The results of the analysis test showed that there was an effect (DSME) on self-care behavior in patients with type II diabetes mellitus with -valeu = 0.002 <&#945- = 0.05. (DSME) is a program that can be applied to people with type II diabetes mellitus, nurses are expected to use DSME as a health program. Keywords: Diabetes Self Management Education, Self Care, Diabetes Mellitus Share Link | Plain Format | Corresponding Author (Dodik Hartono)

42	Bioinformatics	ABS-153
The Computational Analysis of Antiviral Activity Moringa oleifera Bioactive Compounds Against SARS-CoV-2 Main Protease (Mpro) Artika Muliany Tindaon1, Hendra Susanto1,2, Wira Eka Putra1,2 1 Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, Malang, Indonesia 65145 2 Department of Biotechnology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, Malang, Indonesia 65145 Abstract COVID-19 is a pandemic disease that spreads worldwide caused by the SARS-CoV-2 virus, with a high mortality rate. Indonesia is one of the South East Asia countries facing a severe condition affected by COVID-19. Importantly, Indonesia also has tremendous biodiversity of medicinal plants that have pharmacological properties against viral infection, including Moringa oleifera. This in silico study aims to determine the potential of the bioactive compound within Moringa oleifera against the Main protease (Mpro) protein using molecular docking and the interactions produced by the protein-ligand complex. Notably, the results showed that the three bioactive compounds (N-benzyl, O-Ethyl-4-carbamate, and Genistein) had lower affinity values than control compound (Arbidol). The interactions were dominated by hydrophobic interactions. Hence, the protein-ligand complex formed on the Mpro protein was thought to have the opportunity interfere with the structure and function of Mpro SARS-CoV-2 Keywords: Moringa olleifera- SARCOV2- Mpro protein Share Link | Plain Format | Corresponding Author (Hendra Susanto)

43	Bioinformatics	ABS-158
The Potential of Specific Weak Mustard Bioactive Compounds (Nasturtium montanum Wall) as an Antipyretic Agent against Prostaglandin Synthase 2 (PTGS2) Target Protein by In Silico Dilla Anisa Ikhtira1, a) Fatchur Rohman1, b), Sri Rahayu Lestari1, c) 1Department of Biology Postgraduate State University of Malang b)Corresponding author: fatchur.rohman.fmipa[at]um.ac.id a)anisaikhtira123[at]gmail.com c)srirahayulestari[at]um.ac.id Abstract Prostaglandin synthase-2 (PTGS2), which is produced by the arachidonic acid (AA) route and metabolized by the enzyme cyclooxygenase 2 (COX2), is the enzyme that regulates fever, Prostaglandin E2 (PGE2) is produced when the COX-2 enzyme is inhibited, which lowers body temperature. The primary goal of treating fever is PTGS2 suppression using natural substances. This study uses molecular docking to estimate the toxicity and pharmacokinetic characteristics of mild mustard compounds in order to assess their ability to inhibit PTGS2. Using pyRix, simulated docking were carried out, and pyMOL and BIOVIA Discovery Studio were used to show the results. The findings demonstrated that the value of the free bond energy of the three weak mustard compounds: sinapic acid (&#8722-6,7kcal/mol)- Epiprogoitrin (&#8722-6,5kcal/mol) - and Sinigrin(&#8722-5,7kcal/mol)were lowe rcompared to Ibuprofen (&#8722-5,5Kcal/mol). Based on the type of chemical bond, the three compounds^ bonds were stronger than those of the control compounds. According to the ADMET test, the three chemicals were efficiently absorbed and dispersed by the digestive system and did not produce liver damage. The three compounds are suitable as low-toxicity oral medication options for the treatment of fever. Keywords: Bioactive Compounds, Antipyretic Agent, Prostaglandin Synthase 2, In Silico Share Link | Plain Format | Corresponding Author (Dilla Anisa Ikhtira)

44	Biomaterial Science	ABS-9
Survival Analysis of COVID-19 Patients Using Cox Proportional Hazard Regression At Djojonegoro Regional Public Hospital Temanggung Susilowati Gusinta and Jerry Dwi Trijoyo Purnomo Institut Teknologi Sepuluh Nopember Surabaya Abstract In 2019, a new type of virus was identified in Wuhan City, Hubei Province, China, namely COVID-19. Central Java is one of the five provinces with the highest number of additional COVID-19 cases in Indonesia. This study aims to determine the factors that influence the recovery rate of COVID-19 patients using Cox Proportional Hazard regression. It was concluded that on day 0 to day 3 the survival curve fell rapidly with the chance of not recovering in confirmed COVID-19 patients ranging from 1 to 0.5. Based on the modeling carried out, it was found that variables that significantly affect the recovery rate of confirmed COVID-19 patients are respiratory support, number of comorbidities, COVID-19 vaccination status, treatment and epidemiology of the source of transmission. Patients with confirmed COVID-19 who received respiratory support had a recovery rate of 1,771 times faster than confirmed patients with COVID-19 who did not receive respiratory support. Confirmed COVID-19 patients who have received COVID-19 dose 2 have a cure rate of 1,5803 times faster than confirmed COVID-19 patients who have not received COVID-19 vaccination. Confirmed COVID-19 patients who do not have a comorbid disease have a cure rate of 2.037 times faster than confirmed COVID-19 patients who have one comorbid disease and 5.385 times faster than confirmed COVID-19 patients who have two comorbid diseases. Confirmed COVID-19 patients who were not given treatment had a recovery rate of 3,488 times faster than confirmed COVID-19 patients who were given treatment with three types of drugs. Confirmed COVID-19 patients who got COVID-19 after traveling or living in areas with local transmission has a healing rate of 1.5803 times faster than confirmed COVID-19 patients who do not have a history of traveling or living in places at risk of transmission. Keywords: Survival Analysis, COVID-19, Rate of Recovery, Cox Proportional Hazard Regression Share Link | Plain Format | Corresponding Author (Susilowati Gusinta)

45	Biomaterial Science	ABS-57
MICROCRYSTALLINE CELLULOSE FROM OIL PALM EMPTY FRUIT BUNCHES: CORRELATION OF CRYSTALLINITY AND WATER ABSORPTION CAPACITY S. Susi 12a), M. Ainuri 3b), W. Wagiman 3) and M.A. F. Falah 3) 1) Doctoral Programme of Agroindustrial Technology, Faculty of .Agricultural Technology Universitas Gadjah Mada Jl. Flora No 1 Bulaksumur Yogyakarta, Indonesia, 55281 2) Department of Agroindustrial Technology, Faculty of Agriculture, Lambung Mangkurat University, Banjarbaru Jl A Yani KM 36 Banjarbaru Kalimantan Selatan 70714 3) Department of Agroindustrial Technology, Faculty of Agricultural Technology, Gadjah Mada University, Jl. Flora No 1 Bulaksumur Yogyakarta, Indonesia, 55281 Abstract Oil palm empty fruit bunches are one of the most abundant palm oil industrial wastes. OPEFB contains high cellulose which has the potential to be converted into its derivative products, one of which is microcrystalline cellulose. Microcrystalline cellulose (MCC) is purified cellulose and partially depolymerized cellulose, containing both crystalline and amorphous regions. MCC was used as a biofiller and viscosity controlling agent. The characteristics of MCC depend on the nature of the fiber and the appropriate hydrolysis conditions such as acid concentration, temperature, and reaction time. HCl was widely used to modify the MCC crystallinity. The change in crystallinity will correlate with the acid concentration and duration of hydrolysis. The research examined the correlation between MCC crystallinity and its water absorption capacity. Microcrystalline cellulose is produced with variations in HCl concentration and hydrolysis periods. The results showed that the crystallinity index change was not always linear with changes in hydrolysis periods. At a certain hydrolysis periods and concentration of HCl will give high crystallinity. The hydrolysis of 1.5N HCl will give a low crystallinity index if compared to 2.5 and 3.5N HCl. There is a tendency that the longer the acid hydrolysis will decrease the water absorption capacity. Keywords: OPEFB, microcrystalline cellulose, crystallinity water absorption capacity Share Link | Plain Format | Corresponding Author (SUSI S)

46	Biomaterial Science	ABS-72
Study of Antioxidant of Self Nano Emulsifying Drug Delivery System (SNEDDS) Based on Fe3O4/Ginger Extract/VCO Ferrofluid Sri Ainaiyah Ariadi Putri1, sunaryono1,2*, Nasikhudin1, Nadiya Miftachul Chusna1 State University of Malang Abstract In this research, the synthesis of SNEDDS based on Fe3O4/Ginger Extract has been successfully using the coprecipitation method and VCO as the surfactant of the ferrofluids. The characterization sample was carried out using the XRD, FTIR, PSA instrument, and antioxidant activity. The XRD instrument shows the X-ray diffraction pattern of the Fe3O4 crystal phase and ginger extract amorph phase. The results of characterization using FTIR showed that the functional groups of SNEDDS based on Fe3O4/Ginger Extract/VCO ferrofluids were formed with a Zeta Average value of 14.72 nm based on the results of characterization using PSA. Antioxidant activity characterization of the SNEEDS shows that the sample has excellent antioxidant activity. Keywords: Fe3O4, SNEDDS, Ginger, VCO, Ferrofluid Share Link | Plain Format | Corresponding Author (Sri Ainaiyah Ariadi Putri)

47	Biomaterial Science	ABS-102
Crystallinity And Function Group of Nanocomposite BC/CuO With Various Graphene Jibril Maulana(a), Heru Suryanto(b*), Aminnudin(b) a) Master Program of Mechanical Engineering, Graduate School, Universitas Negeri Malang, Jl. Semarang 5, Malang, East Java, Indonesia b) Center of Excellence for Cellulose Composite (CECCom), Department of Mechanical Engineering, Universitas Negeri Malang, Jl. Semarang 5, Malang 65145, East Java, Indonesia *heru.suryanto.ft[at]um.ac.id Abstract Bacterial cellulose (BC) is composed of straight-chain glucose polymers that bonded at the &#946--1,4 position. Bacterial cellulose has three-dimensional structural matrix formed by hydrogen bonds. Graphene can increase the ability of CuO and stability of BC/CuO. Graphene has large specific surface area and also can affect crystallinity and molecular bonding of BC. Objective of this research is investigate effect of addition various graphene (10, 30, and 50%wt CuO) on the BC/CuO nanocomposites properties. Methods synthesis BC was using pineapple peel extraxt in fermentation for 10 days. BC was homogenized by High-Pressure Homogenizer (HPH). An ultrasonic homogenizer was used to mix BC/CuO/Graphene composite solution, then dried using freeze dry to make porous membrane. Membrane were analyzed using XRD and FTIR to determine effect of graphene addition on the crystallinity and functional groups of BC/CuO. Results show that addition of CuO and graphene decreasing crystallinity of BC/CuO/ Graphene, which is adding more graphene make lower peak from control (88.85%) until BC/CuO with addition graphene 50% (79.78%) of Cr. The FTIR results indicated the addition of graphene can affect on O-H bonds and increasing C-C bond have been see changing number of transmittances in the range of 3400 cm-1 and 1172 cm-1. Keywords: Bacterial cellulose, Graphene, Copper Oxide, XRD, FTIR Share Link | Plain Format | Corresponding Author (Heru Suryanto)

48	Biomaterial Science	ABS-108
Mechanical Properties Analysis of Pineapple Leaf Fiber with Various TEMPO Oxidation Level Jovan Hilmansyah R(1), Heru Suryanto(2*), Aminnudin(2), Yanuar Rohmat A P(2), J S Binoj(3), Jibril Maulana(2), Rahmad Ikrom R(1) 1)Bachelor Program of Mechanical Engineering, Malang State University, Jl. Semarang 5, Malang 65145, Indonesia. 2)Center of Excellence for Cellulose Composite (CECCom), Mechanical Engineering Department, Malang State University, Jl. Semarang 5, Malang 65145, Indonesia. *heru.suryanto.ft[at]um.ac.id 3)Institute of Mechanical Engineering, Saveetha School of Engineering, Saveetha Institute of Medical and Technical Sciences (SIMATS), Tamilnadu 602105, India. Abstract Pineapple Leaf Fiber (PALF) known some advantages namely renewable, noncytotoxic, and excellent mechanical properties. However, mechanical properties of PALF could be improved using chemical treatment. This study aims to analysis the mechanical properties of PALF after treated by 2,2,6,6 Tetramethylpiperidinyloxy (TEMPO). The methods were, firstly, alkalization process using 6 percent Sodium Hydroxide (NaOH) to remove impurities from PALF. Alkalized PALF then treated with TEMPO at concentration of 1, 2, 3 mM. Treated fibers were dried in the oven at 60C for 4 h. Mechanical properties were analized using Single Fiber Tensile Tester. Results indicates that oxidation treatment could affect the mechanical properties of PALF. Each sample namely PALF, Alkalized PALF, and oxidated PALF at the concentration of 1, 2 and 3 mM have tensile strength of 230, 496, 1167, 732 and 901 MPa respectively. Differences occurs due to various total area of samples. Modulus Young of alkalized PALF, TEMPO at 1, 2, 3 mM of concentration by 6.4, 17.2, 18.1 and 22.7 GPa respectively. Modulus Young escalation affected by fibrillation after treatments that made each surface of fibers expand and strengthen the bond of each individual fibrils. The loss of surface layer also influenced the tensile strength of treated PALF. Keywords: PALF, TEMPO, Oxidation, Fibrillation, Mechanical Properties. Share Link | Plain Format | Corresponding Author (Heru Suryanto)

49	Biomaterial Science	ABS-148
Hydrochar from Hydrothermal Carbonization Process of Galam Bark Waste (Malaleuca cajuputi) as Sasirangan Waste Adsorbent Risaldi Ridwan, Hanna Habibah, Desmalina Safitri, Sunardi 1Chemistry Department, Faculty of Mathematics and Natural Sciences, Lambung Mangkurat University, Banjarbaru, Indonesia 2Ecomaterial Research Group, Faculty of Mathematics and Natural Sciences, Lambung Mangkurat University, Banjarbaru, Indonesia 3Wetland-Based Materials Research Center, Lambung Mangkurat University, Banjarbaru, Indonesia Abstract Galam wood (Malaleuca cajuputi) is one of the woody plants that can grow well in the peat swamp environment in South Kalimantan. Research on the production of hydrochar by hydrothermal carbonization process of galam bark for adsorption of sasirangan waste has been carried out. In this study, the influence of processing time of the hydrothermal carbonization on the yield and characteristics of hydrochar was investigated. It was found that the processing time of hydrothermal carbonization is the important factor influencing the hydrochar production. The results of FTIR analysis demonstrated that hydrochar from galam bark had different functional group, which indicated had different characters. The surface morphology of hydrochar and galam bark also showed significant different based on scanning electron microscope (SEM) analysis. The results also showed that hydrochar produced at the longer processing time had significantly higher sasirangan waste adsorption capacity. Keywords: hydrothermal carbonization, hydrochar, galam bark waste, sasirangan waste, adsorbent Share Link | Plain Format | Corresponding Author (Risaldi Ridwan)

50	Biomedical Science	ABS-2
Hippocampal Volume Change in Multiple Sclerosis Patients Ibnu Diptya Prawatya (a*), Ahmad Bayhaqi (b), Yuyun Yueniwati (b) a) Master of Biomedical Sciences, Faculty of Medicine, Brawijaya University, Malang, Indonesia *diptya.ibnu[at]gmail.com b) Department of Radiology, Saiful Anwar General Hospital, Malang, Indonesia Abstract Multiple Sclerosis (MS) is a clinical disease which attribute to decrease long-term memory retrieval impairment associated with the hippocampus. Many studies of MS have involved hippocampus in which characterized pathologically by demyelination, nerve damage, and synapse loss. This study aims to determine changes in hippocampal volume in patients who have been diagnosed with MS. Retrospective study was conducted where hippocampal volume change of patients diagnosed with MS were calculated by using Magnetic Resonance Imaging (MRI). MRI of the hippocampus was performed with a power of 3 Tesla units (PHILIPS INGENIA 3.0T) with a 20-channel head coil, then hippocampal volumetric analysis data was performed using Hippocampus Subfield Segmentation (HIPS) on Volbrain software. Volume changes in hippocampus had several results with no significant relationship between gender and the appearance of brain atrophy (p>0.05). Meanwhile, a significant relationship was found to the overall age- both age without atrophy and age with atrophy (p<0.05). From this study, it was shown that there were volume changes in patients diagnosed with Multiple Sclerosis in all ages, either with or without atrophy. The field of radiology plays important role where hippocampal volume changes can occur in patients with Multiple Sclerosis. Keywords: Neuroimaging- MRI- Hippocampus Share Link | Plain Format | Corresponding Author (Ibnu Diptya Prawatya)

51	Biomedical Science	ABS-5
Light Exposure^s Effects on Inactive Frequency and Distance Moved of Zebrafish in Insomnia Model Dheka Sapti Iskandar,Zamroni Afif, Husnul Khotimah, Irawan Satriotomo, Shahdevi Nandar Kurniawan, Annisatul Hakimah, Syafiatul Azizah, Nurvia Andriani, Kartika Agustina Brawijaya University Abstract Insomnia as inability to maintain or elevate sleep quality at least three times a day, more than three days in a week, or more than three months. One of theories explaining insomnia causes focuses on a condition named hyperarousal, which is related to increasing mental, cognitive, and motor activities. This study is applied to insomnia model, that zebrafish larvae were exposed to a lamp with intensity of 200 lux. There were four phases of light exposure, are 12 hours of light, 12 hours of dark, 18 hours of light, and 6 hours of flash. The flash phase was divided into two, namely 2 minutes light (on) 2 minutes dark (off) and 4 minutes light 1 minute dark. The result is that the 24-hour treatment, both light and dark cycles, have a lower Inactive Frequency and a higher distance moved compared to the control group. Keywords: Insomnia, Zebrafish, Inactive Frequency, Distance moved. Share Link | Plain Format | Corresponding Author (Dheka Sapti Iskandar)

52	Biomedical Science	ABS-19
Betatrophin: The Long-Term Exploration of Potential Biomarker for Metabolic Syndrome and Cancer-Related Metabolic Perturbation Hendra Susanto 1 Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, Indonesia 2 Department of Biotechnology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, Indonesia Abstract The clinician and research community focus on the increasing global prevalence of metabolic syndrome and cancer. Prevention of the high incidence of these two pathological conditions is urgent to suppress the progression of mortality in patients. Therefore, the exploration of potential biomarkers that have a significant correlation to the two events was crucial. Betatrophin, a liver-derived hormone, plays a pivotal role in the pathogenesis of hypertriglyceridemia, metabolic disorders, and cancer development. The in vivo and the clinical study was done to characterize betatrophin activity in several diseases. In the last decade, it has been reported that this hormone significantly contributes to metabolic syndrome, obesity, diabetes mellitus, diabetic nephropathy, pancreatic ductal adenocarcinoma, hepatocellular carcinoma, and colorectal carcinoma. Furthermore, the animal model and clinical investigation proved that the significant alteration of betatrophin may contribute to those several pathogenic conditions. Betatrophin may induce lipid metabolism disorder and trigger physiological and cellular changes. Importantly, on the other hand, physical activity intervention with moderate-intensity exercise treatment also decreased the circulating level of betatrophin in subjects with metabolic syndrome symptoms. Hence, the future exploration of this liver potential hormone as a serological biomarker for metabolic syndrome and cancer-linked metabolic disorders could benefit clinical studies Keywords: Betatrophin, Biomarker, Metabolic Syndrome, Cancer Share Link | Plain Format | Corresponding Author (Hendra Susanto)

53	Biomedical Science	ABS-39
The Expression of Metastases and Liver Damage-Related Gene Among Indonesian Colorectal Cancer Liver Metastases Patients Ainul Mardiah 1, Ayu Febrinanti 1, Shabrina Andira Putri 1, Hendra Susanto1,2a), Adeodatus Yuda Handaya3,4b), Moch. Sholeh 1 Department of Biology , Faculty of Mathematics and Natural Science, Universitas Negeri Malang, Indonesia Department of Biotechnology, Faculty of Mathematics and Natural Science, Universitas Negeri Malang, Indonesia Faculty of Medicine, Universitas Gadjah Mada, Indonesia Divison of Digestive Surgery, Department of Surgery, Dr. Sardjito Hospital, Yogyakarta, Indonesia Abstract Colorectal cancer (CRC) is the third most common cancer and the second cancer with the highest mortality rate in the world. The main cause of death in colorectal cancer patients is cancer that has metastasized with the most common site of metastasis being the liver. EMT is an important mechanism of metastasis related to the malignancy of cancer cells and regulated by transcription factor, Slug. EMT also regulated by TGF-&#946- signaling pathway and studies revealed that TGF-&#946-R1 significantly associated with the risk of developing CRC in humans. Damaged liver can interfere liver function in producing various regulatory proteins, such as Angiopoietin-Like Protein 8 (ANGPTL8). This study aims to detect the expression of the Slug, TGF-&#946-R1, ANGPTL8 gene in liver metastases originating from colorectal cancer through mRNA level analysis with RT-qPCR method and analyzed descriptively. The results showed Slug and TGF-&#946-R1 expression in CRC metastatic liver tissue of patients at stage IVC was higher than stage IVB and the expression both gens found higher in male than female patients. The ANGPTL8 gene expression also found higher in the group with higher AST and ALT levels. Based on the results, it is suggested that Slug, TGF-&#946-R1, ANGPTL8 expression correlated with CRC liver metastases. Further research is needed to determine the regulation this gene in colorectal cancer metastatic organs in driving the incidence of subsequent metastases and their implications for colorectal cancer severity. Keywords: Colorectal cancer- Liver metastasis- Slug- TGF-&#946-R1- ANGPTL8 Share Link | Plain Format | Corresponding Author (Ainul Mardiah)

54	Biomedical Science	ABS-42
Identification of Salivary Microflora in Residents of Medan Suburban Area Dina Rahmi Solihad Nasution a), Zulham Yamamoto b) a) Master^s Program in Biomedical Science, Faculty of Medicine, Universitas Sumatera Utara Jalan Dr T Mansur 5, Medan 20155 *dinarahmisolihadn[at]gmail.com b)Department of Histology, Faculty of Medicine, Universitas Sumatera Utara Abstract Saliva as a source of human DNA has many advantages over other DNA sources such as being noninvasive and stable. Preservation of saliva as a source of DNA has challenges since salivary microflora has the potential to degrade the DNA of the human genome. This study aims to identify salivary microflora (protozoa, bacteria, and yeast) in residents of Medan suburban area, North Sumatra. This is a cross-sectional study. A total of 30 subjects were recruited using convenience sampling. A total of 30 saliva DNA samples from each subject were extracted using spin column method. Identification of protozoa was carried out using a PCR approach through Trichomonas tenax and Entamoeba gingivalis gene amplification. Identification of bacteria and yeast was carried out using microbiological and biomolecular approaches. A colony PCR targeted the 16S rRNA gene (bacteria) and ITS C. albicans (yeast). Saliva samples in residents of Medan suburban area contain T. tenax and E. gingivalis, as well as positive Gram bacteria-cocci. Catalase tests showed that 83% of bacterial samples were Staphylococcus while the rest were Streptococcus. In the yeast group, there is Candida albicans. Mann Whitney U Test showed that age has association with T. tenax (p<0.05) and C. albicans (p<0.05). Keywords: saliva, suburban, microflora, yeast, bacteria Share Link | Plain Format | Corresponding Author (Dina Rahmi Solihad Nasution)

55	Biomedical Science	ABS-64
The Effect of Mesenchymal Stem Cells in Systemic Lupus Erythematosus: A Systematic Review Nurhasan Agung Prabowo, Arief Nurudhin, Desy Puspa Putri, and Yulyani Werdiningsih 1.Faculty of Medicine, Universitas Sebelas Maret, Ir Sutami Street No 36, Kentingan, Jebres, Surakarta, Indonesia, 57126 2.Universitas Sebelas Maret Hospital, Ahmad Yani Street No 200, Kartasura, Sukoharjo, Indonesia, 57161 3.Doctoral Degree of Medical Science Program, Faculty of Medicine, Universitas Sebelas Maret, Ir Sutami Street No 6, Kentingan, Jebres, Surakarta, 57126 Abstract SLE is an autoimmune illness affecting various organs and tissues. MSCs (mesenchymal stem cells) are a promising treatment option for autoimmune and haematological diseases. The effectiveness and mechanisms of MSC treatment for SLE will be summarized in this review. Therefore, this paper aims to review mesenchymal stem cells in SLE. We looked through Pubmed, Cochrane, and Scopus to find peer-reviewed studies that looked at how mesenchymal stem cells affect SLE. The research included in this review was a study from 2000 to 2022, a randomized control trial using intervention mesenchymal stem cells, the full text of the article can be obtained, and the patient^s age is over 18 years. Three reviewers independently extracted data on relevant clinical outcomes, trial characteristics, and patient characteristics and used the Cochrane Risk of Bias Assessment Tool. The certainty of the evidence was assessed using the Grading of Recommendations Assessment, Development, and Evaluation. In SLE patients, MSC treatment can lower anti-dsDNA, antinuclear antigen (ANA), proteinuria, and serum creatinine. To ameliorate the lesions in SLE, MSCs can block inflammatory factors like MCP-1 and HMGB-1, as well as inflammation-related signalling pathways including the NF-B, JAK/STAT, and Akt/GSK3 signalling pathways Keywords: Messenchymal stem cell, Systemic Lupus Erythematosus Share Link | Plain Format | Corresponding Author (Nurhasan Agung Prabowo)

56	Biomedical Science	ABS-65
COVID-19 Vaccine Safety and Efficacy in Systemic Lupus Erythematosus: A Systematic Review Nurhasan Agung Prabowo, Tonang Dwi Ardyanto, Risalina Myrtha, Laily Shofiyah 1.Faculty of Medicine, Universitas Sebelas Maret, Ir Sutami Street No 36, Kentingan, Jebres, Surakarta, Indonesia, 57126 2.Universitas Sebelas Maret Hospital, Ahmad Yani Street No 200, Kartasura, Sukoharjo, Indonesia, 57161 3.Doctoral Degree of Medical Science Program, Faculty of Medicine, Universitas Sebelas Maret, Ir Sutami Street No 6, Kentingan, Jebres, Surakarta, 57126 Abstract SLE is a kind of autoimmune rheumatic illness that can range in severity and organ involvement. Immune dysregulation is a feature of SLE, which is exacerbated by immunosuppressive drugs. Most live vaccines are contraindicated in SLE, and inactivated vaccines must be given before immunosuppression. Therefore, this paper aims to review COVID-19 vaccine safety and efficacy in Systemic Lupus Erythematosus. We systematically searched Pubmed, Cochrane, and Scopus for peer-reviewed studies examining the COVID-19 vaccine safety and efficacy in Systemic Lupus Erythematosus. The research included in this review was a study from 2000 to 2022, the full text of the article can be obtained, and the patient^s age is over 18 years. Three reviewers independently extracted data on relevant clinical outcomes, trial characteristics, and patient characteristics and used the Cochrane Risk of Bias Assessment Tool. The certainty of the evidence was assessed using the Grading of Recommendations Assessment, Development, and Evaluation. COVID-19 vaccinations tend to be safe in SLE patients, whereas mRNA vaccines cause more significant local responses. The adverse effects were mildlocal soreness, weariness, and a headache. The chances of a significant lupus flare are slim. COVID-19 immunisation is less efficient than in the general population, especially in individuals on immunosuppressive medications or using inactivated vaccines. Keywords: COVID-19 Vaccine, Safety, Efficacy, Systemic Lupus Erythematosus Share Link | Plain Format | Corresponding Author (Nurhasan Agung Prabowo)

57	Biomedical Science	ABS-68
Anti-oxidant Effect of Phyllanthus niruri and Catharanthus roseus Combination Extract on Breast Cancer Mice Models Sefihara Paramitha Sakti (a), Fikriya Novita Sari (a), Farida Rachmawati (a), Muhaimin Rifai(a*) a) Biology Department, Faculty of Mathematics and Natural Sciences, Brawijaya University, Jl. Veteran Malang 65145, East Java, Indonesia *immunobiology[at]ub.ac.id Abstract Breast cancer is one type of cancer that has the highest prevalence and high mortality rate. Recent research reported that breast cancer may have an effect on the physiology of other organ, such as oxidative stress condition in the liver. Beside that, some agents such as meniran (Phyllanthus niruri) and Madagascar perwinkle (Catharanthus roseus) appear to maintain redox homeostasis. This study aims to investigate the effectiveness combination of Phyllanthus niruri and Catharanthus roseus extract (PCE) in modulating oxidative stress based on the levels of malondialdehyde (MDA), superoxide dismutase (SOD), and Nuclear factor erythroid 2-related factor 2 (Nrf2). Experimental animals were divided into six different groups- healthy control (normal), cancer (DMBA), cancer mice with cisplatin administration, cancer mice with PCE administration with three different dose, including dose 1 (500 mg/kg PN+15 mg/kg CR), dose 2 (1000 mg/kg PN+75 mg/kg CR), and dose 3 (2000 mg/kg PN+375 mg/kg CR). The mice were injected with DMBA once a week for six weeks to induce cancer in mice. PCE was orally administrated to breast cancer mice for 14 days. The levels of MDA, Nrf2, and SOD were analyzed by flow cytometry. The results showed that breast cancer induction using DMBA increased the level of MDA and Nrf2, but decreased the level of SOD significantly compared to the normal group (p<0.05). In certain dose, administration of PCE was able to reduce MDA, Nrf2 levels and increase the level of SOD significantly (p<0.05). The PCE has potency as alternative therapeutic agent to modulate redox homeostasis in breast cancer by suppressing MDA and Nrf2 levels, also increasing SOD levels. Keywords: Breast cancer, Catharanthus roseus, Phyllanthus niruri, anti-oxidant. Share Link | Plain Format | Corresponding Author (Muhaimin Rifai)

58	Biomedical Science	ABS-73
The potency of Ruellia tuberosa L. leaf decoction as antidiabetic: an in-silico study and antioxidant profile Ekowati Retnaningtyas (a*), Budi Susatia (a), Muhammad Hasan Wattiheluw (a), Yuslinda Annisa (b), Achmad Rudijanto (c) (a) Politeknik Kesehatan Kemenkes, Jalan Besar Ijen no. 77C 65112, Malang, East Java, Indonesia * ekowati_retnaningtyas[at]poltekkes-malang.ac.id (b) Department of Biology, Faculty Mathematics and Natural Science, Universitas Negeri Malang, Malang, East Java, Indonesia (c) Department of Internal Medicine, Faculty of Medicine, Brawijaya University, Malang, Indonesia Abstract Diabetes mellitus (DM) is a group of metabolic diseases characterized by impaired insulin secretion and insulin resistance which can cause various complications. Ruellia tuberosa L. (RTL) is one of the plants that are commonly found in the community. RTL has a role as an antioxidant, anti-inflammatory, anticancer, and other physiological abilities. This study aimed to evaluate the potential of RTL as an anti-diabetic through molecular docking and to test the antioxidant activity of RTL. The methods used include identification of RTL active compounds, selection of target proteins in various diabetes pathways, prediction of active sites, docking and visualization, and antioxidant activity test. The results of LC-HRMS showed that the RTL extract contained several main compounds, including betaine, choline, caffeic acid, and scrophulein. Molecular docking shows that the active compounds that have the most potential as inhibitors that compete with DM drug compounds are Scrophulein. These results were obtained from the comparison of the binding energy values of the two active compounds which could be lower than the binding energy values of the drug compounds. The results of the antioxidant activity test showed that the RTL has high antioxidant activity and has the potential to suppress free radicals in DM. Keywords: Ruellia tuberosa L.- decoction- antidiabetic- in-silico- antioxidan Share Link | Plain Format | Corresponding Author (Ekowati Retnaningtyas)

59	Biomedical Science	ABS-75
The Effect Of Mesenchymal Stem Cell Secretome On The Levels Of Anti-Dsdna Antibodies And Complement C3, Histological Features of Pulmonary Vascular And Kidney in Lupus Mice Models Arief Nurudhin, Nurhasan Agung Prabowo, Zainal Arifin Adnan, Indrayana Faculty of Medicine, Universitas Sebelas Maret, Ir Sutami Street No 36, Kentingan, Jebres, Surakarta, Indonesia, 57126 Abstract Introduction: No definitive treatment is available for SLE. Mesenchymal stem cell secretome is one of promising novel treatments in SLE. Our study was aimed to identify the effect of mesenchymal stem cell secretome on the levels of anti-dsDNA antibodies and complement c3, histological features of pulmonary vascular and kidney in lupus mice models. Methods: The subjects were 21 female mice of Mus musculus Balb/C strain, which were categorized into 3 groups of 7 mice, i.e. the control group receiving 0.5 cc of 0.9% NaCl injection and 0.45 cc of 0.9% NaCl intraperitoneal injection, the lupus group receiving 0.5 cc of pristane injection and 0.45 cc of 0.9% NaCl intraperitoneal injection, and the treatment group receiving 0.5 cc pristane injection and 0.45 cc of secretome intraperitoneal injection. Results: The study showed that there was a difference on the levels of anti-dsDNA antibodies level among the three groups. After a continuation, there were also differences on the level of complemen C3, histological features of pulmonary vascular, and histological features of kidney. Conclusion: The study demonstrates the effect of mesenchymal stem cell secretome on reduced level of anti-dsDNA antibodies and increased levels of complement c3, improved histological features of pulmonary vascular and kidney in lupus mice models. Keywords: Mesenchymal stem cell secretome- anti-dsDNA antibodies- Complement C3- histological features of pulmonary vascular- histological features of kidney- Lupus Share Link | Plain Format | Corresponding Author (Nurhasan Agung Prabowo)

60	Biomedical Science	ABS-83
The Gut Microbiota in Undernutrition : A Preliminary Study Yunita Rakhmawati, Nur^aini Kartikasari UM Abstract Malnutrition in Indonesia is still a problem that must be considered to achieve sustainable development goals in overcoming all forms of malnutrition. One alternative is to pay attention to the health of the gastrointestinal tract of people with malnutrition through the gut microbiota. This study compares the gut microbiota of normal rats and malnourished rats. This research was conducted with an experimental study. The rats were treated with a standard diet and PEM for four weeks. Afterward, body weight and gut microbiota were analyzed from stool samples. Microbiota data analyzed were the number and groups of Lactobacillus, Bifidobacterium, Enterobacter, and E. Coli bacteria. Keywords: Gut microbiota, malnutrition Share Link | Plain Format | Corresponding Author (Luthfi Syarifa Rasyidatu Hasnanda)

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