The Effect of Allium sativum, Curcuma mangga, and Acorus calamus Nanoparticles to Profile of Mice Leukocytes Induced by Cisplatin
Bayyinatul Muchtaromah (a*), Farah Fauzia Maulahibati (a), Prilya Dewi Fitriasari (a), Mujahidin Ahmad (a), Eko Budi Minarno (a), and Kenji Mishima (b)

(a) Department of Biology, Faculty of Science and Technology, The State Islamic University of Maulana Malik Ibrahim Malang, Jl. Gajayana 50, Malang, Indonesia
*bayyinatul[at]bio.uin-malang.ac.id
(b) Department of Chemical Engineering, Faculty of Engineering, Fukuoka University, Japan

Abstract

The induction of cisplatin can increase the production of reactive oxygen species (ROS) and cause an imbalance in the immune system. This condition can be overcome with use of A. sativum, C. mangga, and A. calamus. That plants contain several active compounds to improve the function of the immune system, including leukocytes. This study aimed to know the effect of nanoparticles A. sativum, C. mangga, and A. calamus on the total cell leukocyte and total leukocyte types (neutrophil, basophil, eosinophil, lymphocyte, and monocyte) in mice that induced by cisplatin. This study used the Complete Random Design (CRD) with 5 treatments and 6 replicates. The examination of the total leukocyte used a hemocytometer and the total leukocyte types used a blood smear. The results showed that there was an effect of giving nanoparticles A. sativum, C. mangga, and A. calamus on the total leukocyte, neutrophils, and lymphocytes mice and there was no effect of giving nanoparticles A. sativum, C. mangga, and A. calamus to basophils and eosinophils mice that are induced by cisplatin. The dose of nanoparticles extracts A. sativum, C. mangga, and A. calamus 25 mg/kgBW and 50 mg/kgBW had a significant effect compared to the other treatment groups.

Keywords: Acorus calamus, Allium sativum, Cisplatin, Curcuma mangga, Leukocytes, Nanoparticles

Topic: Bionanotechnology