Characterization and Effectiveness Test of Single Garlic Extract-loaded Chitosan-Alginate Nanoencapsulation to Hemolysis Percentage
Dahniar Nur Aisyah (a), Sri Rahayu Lestari (a*), Siti Imroatul Maslikah (a), Sunaryono (b)

a) Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, Jl. Semarang 5, Malang 65145, Indonesia
*srirahayulestari[at]um.ac.id
b) Department of Physics, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, Jl. Semarang 5, Malang 65145, Indonesia

Abstract

Single garlic grows as a result of an unsuitable growing environment. Single garlic has higher active compound content than normal cloves of garlic. One of the active compounds is allicin. Allicin causes hemolysis when in the bloodstream. Chitosan-alginate nanoencapsulation (CANe) is a delivery system that is able to protect the active compounds in single garlic extract (SGE), so it is suspected that it can minimize the degradation of the active compounds and also reduce the hemolysis. This study aims are for characterizing and test the effectiveness of SGE-CANe on the hemolysis percentage. This research method were single garlic extraction, preparation of SGE-CANe, characterization of SGA-CANe (particle size, polydispersity index, zeta potential), and in-vitro SGE-CANe hemocompatibility test using the blood of Balb/C mice. The results showed that the SGE-CANe droplet size was 438,6 nm- SGE-CANe polydispersity index (PdI) value was 0,446- the zeta potential of SGE-CANe was -24,5 mV- and the hemolysis percentage of SGE-CANe (3,05%) < SGE (8,95%). The particle size and PdI of SGE-CANe have reached the research criteria, which is nanometer-sized particles and homogeneously distributed, but the zeta potential value is low. The CANe formulation reduced the hemolysis percentage of SGE in the blood of Balb/C mice.

Keywords: Balb/C mice- Characterization- Chitosan-alginate nanoencapsulation- Hemolysis percentage- Single garlic extract

Topic: Bionanotechnology