The Comparison of TGF-B1, Slug, and Twist Gene Expression in Colorectal Subjects with Hepatic and non-Hepatic Metastatic
Adinda Permatasari1, Ikke Astrid Dewi1, Rifqah Dwi Pratiwi 2, Hendra Susanto1, a), Adeodatus Yuda Handaya3, 4, b), and Moch Sholeh1

1Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, Indonesia
2Department of Biotechnology., Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang Indonesia
3Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia
4Division of Digestive Surgery, Department of Surgery, Dr. Sardjito Hospital, Yogyakarta, Indonesia

a) Corresponding author: hendrabio[at]um.ac.id
b) yudahandaya[at]ugm.ac.id

Abstract

In 2020, GLOBOCAN reported that the incidence of Colorectal Cancer (CRC) in men was in third place and in women it was in second. CRC can be caused by genetic factors and environmental factors. Epidemiological studies suggest an unhealthy lifestyle can be a strong candidate for the development of CRC. The development and metastasis of CRC are characterized by the epithelial-mesenchymal transition (EMT). There are many genes known to be involved in CRC malignancy, including TGF-B1, Slug and Twist. This study aims to determine the differences in TGF-B1, Slug and Twist gene expression in CRC patient with and without liver metastasis. This research was carried out by measuring the expression of all genes using the RT-qPCR method. Importantly, the expression of slug genes in non-hepatic metastasis patients was higher than other patient groups. TGF-B1, Slug and Twist is potential to be used in treatment strategies or predictions for CRC patients related to the significant difference the expression in the two groups of patients. Hence, the development of CRC gene biomarker for early stages detection can be focused on the primary gene regulator-linked metastasis.

Keywords: CRC Hepatic Metastatic- CRC non-Hepatic Metastatic- TGF-B1- Slug- Twist

Topic: Biomedical Science