PHYTOCHEMICAL SCREENING AND SYNTHESIS NANOPARTICLES OF MOJO LEAF EXTRACT (Aegle marmelos) FOR BOTANI PESTICIDES Sofia Ery Rahayua), Fatchur Rohman, Rahmi Masita, and Bagus Priambodo
Department of Biology, Faculty of Mathematics and Natural Science, Universitas Negeri Malang,
Indonesia
Abstract
The purpose of the study was to determine the chemical composition and synthesis of nanoparticles based of maja (Aegle marmelos) leaf extract. Maja leaves were obtained from Malang, Indonesia. Maja leaf extract was made with methanol and chloroform as solvents. Phytochemical screening of maja leaf extract was analyzed by LCMS. The synthesis nanoparticles of maja leaf extract using chitosan as a coating. The synthesis was carried out by 2 methods without and using ionic gelation. Measurement of nanoparticles was carried out using the Particle Size Analyzer (PSA) at the ILRC Laboratory of the University Indonesia. The results of the study found 108 active compounds in the methanol extract and 92 compounds in the chloroform extract of maja leaves. The total phenol of the maja leaf extract was 2.47 mg/ml and the total flavonoid was 12.31 mg/ml. Synthesis of nanoparticles without using ionic gelation resulted in a polydispersity was 1.7, while using ionic gelation was < 1. Both methods produced particle sizes 800 nm and zeta potential values >30 were quite stable. Based on the results of this study, the compound mixing process is better by adding ionic gelation with sonication because it produces good particle uniformity and is quite stable.