The Expression of Metastases and Liver Damage-Related Gene Among Indonesian Colorectal Cancer Liver Metastases Patients Ainul Mardiah 1, Ayu Febrinanti 1, Shabrina Andira Putri 1, Hendra Susanto1,2a), Adeodatus Yuda Handaya3,4b), Moch. Sholeh 1
Department of Biology , Faculty of Mathematics and Natural Science, Universitas Negeri Malang, Indonesia
Department of Biotechnology, Faculty of Mathematics and Natural Science, Universitas Negeri Malang, Indonesia
Faculty of Medicine, Universitas Gadjah Mada, Indonesia
Divison of Digestive Surgery, Department of Surgery, Dr. Sardjito Hospital, Yogyakarta, Indonesia
Abstract
Colorectal cancer (CRC) is the third most common cancer and the second cancer with the highest mortality rate in the world. The main cause of death in colorectal cancer patients is cancer that has metastasized with the most common site of metastasis being the liver. EMT is an important mechanism of metastasis related to the malignancy of cancer cells and regulated by transcription factor, Slug. EMT also regulated by TGF-β- signaling pathway and studies revealed that TGF-β-R1 significantly associated with the risk of developing CRC in humans. Damaged liver can interfere liver function in producing various regulatory proteins, such as Angiopoietin-Like Protein 8 (ANGPTL8). This study aims to detect the expression of the Slug, TGF-β-R1, ANGPTL8 gene in liver metastases originating from colorectal cancer through mRNA level analysis with RT-qPCR method and analyzed descriptively. The results showed Slug and TGF-β-R1 expression in CRC metastatic liver tissue of patients at stage IVC was higher than stage IVB and the expression both gens found higher in male than female patients. The ANGPTL8 gene expression also found higher in the group with higher AST and ALT levels. Based on the results, it is suggested that Slug, TGF-β-R1, ANGPTL8 expression correlated with CRC liver metastases. Further research is needed to determine the regulation this gene in colorectal cancer metastatic organs in driving the incidence of subsequent metastases and their implications for colorectal cancer severity.
