The potency of Ruellia tuberosa L. leaf decoction as antidiabetic: an in-silico study and antioxidant profile Ekowati Retnaningtyas (a*), Budi Susatia (a), Muhammad Hasan Wattiheluw (a), Yuslinda Annisa (b), Achmad Rudijanto (c)
(a) Politeknik Kesehatan Kemenkes, Jalan Besar Ijen no. 77C 65112, Malang, East Java, Indonesia
* ekowati_retnaningtyas[at]poltekkes-malang.ac.id
(b) Department of Biology, Faculty Mathematics and Natural Science, Universitas Negeri Malang, Malang, East Java, Indonesia
(c) Department of Internal Medicine, Faculty of Medicine, Brawijaya University, Malang, Indonesia
Abstract
Diabetes mellitus (DM) is a group of metabolic diseases characterized by impaired insulin secretion and insulin resistance which can cause various complications. Ruellia tuberosa L. (RTL) is one of the plants that are commonly found in the community. RTL has a role as an antioxidant, anti-inflammatory, anticancer, and other physiological abilities. This study aimed to evaluate the potential of RTL as an anti-diabetic through molecular docking and to test the antioxidant activity of RTL. The methods used include identification of RTL active compounds, selection of target proteins in various diabetes pathways, prediction of active sites, docking and visualization, and antioxidant activity test. The results of LC-HRMS showed that the RTL extract contained several main compounds, including betaine, choline, caffeic acid, and scrophulein. Molecular docking shows that the active compounds that have the most potential as inhibitors that compete with DM drug compounds are Scrophulein. These results were obtained from the comparison of the binding energy values of the two active compounds which could be lower than the binding energy values of the drug compounds. The results of the antioxidant activity test showed that the RTL has high antioxidant activity and has the potential to suppress free radicals in DM.
