Identification of Salivary Microflora in Residents of Medan Suburban Area Dina Rahmi Solihad Nasution a), Zulham Yamamoto b)
a) Master^s Program in Biomedical Science, Faculty of Medicine, Universitas Sumatera Utara
Jalan Dr T Mansur 5, Medan 20155
*dinarahmisolihadn[at]gmail.com
b)Department of Histology, Faculty of Medicine, Universitas Sumatera Utara
Abstract
Saliva as a source of human DNA has many advantages over other DNA sources such as being noninvasive and stable. Preservation of saliva as a source of DNA has challenges since salivary microflora has the potential to degrade the DNA of the human genome. This study aims to identify salivary microflora (protozoa, bacteria, and yeast) in residents of Medan suburban area, North Sumatra. This is a cross-sectional study. A total of 30 subjects were recruited using convenience sampling. A total of 30 saliva DNA samples from each subject were extracted using spin column method. Identification of protozoa was carried out using a PCR approach through Trichomonas tenax and Entamoeba gingivalis gene amplification. Identification of bacteria and yeast was carried out using microbiological and biomolecular approaches. A colony PCR targeted the 16S rRNA gene (bacteria) and ITS C. albicans (yeast). Saliva samples in residents of Medan suburban area contain T. tenax and E. gingivalis, as well as positive Gram bacteria-cocci. Catalase tests showed that 83% of bacterial samples were Staphylococcus while the rest were Streptococcus. In the yeast group, there is Candida albicans. Mann Whitney U Test showed that age has association with T. tenax (p<0.05) and C. albicans (p<0.05).
