The Effect Of Mesenchymal Stem Cell Secretome On The Levels Of Anti-Dsdna Antibodies And Complement C3, Histological Features of Pulmonary Vascular And Kidney in Lupus Mice Models Arief Nurudhin, Nurhasan Agung Prabowo, Zainal Arifin Adnan, Indrayana
Faculty of Medicine, Universitas Sebelas Maret, Ir Sutami Street No 36, Kentingan, Jebres, Surakarta, Indonesia, 57126
Abstract
Introduction: No definitive treatment is available for SLE. Mesenchymal stem cell secretome is one of promising novel treatments in SLE. Our study was aimed to identify the effect of mesenchymal stem cell secretome on the levels of anti-dsDNA antibodies and complement c3, histological features of pulmonary vascular and kidney in lupus mice models. Methods: The subjects were 21 female mice of Mus musculus Balb/C strain, which were categorized into 3 groups of 7 mice, i.e. the control group receiving 0.5 cc of 0.9% NaCl injection and 0.45 cc of 0.9% NaCl intraperitoneal injection, the lupus group receiving 0.5 cc of pristane injection and 0.45 cc of 0.9% NaCl intraperitoneal injection, and the treatment group receiving 0.5 cc pristane injection and 0.45 cc of secretome intraperitoneal injection. Results: The study showed that there was a difference on the levels of anti-dsDNA antibodies level among the three groups. After a continuation, there were also differences on the level of complemen C3, histological features of pulmonary vascular, and histological features of kidney. Conclusion: The study demonstrates the effect of mesenchymal stem cell secretome on reduced level of anti-dsDNA antibodies and increased levels of complement c3, improved histological features of pulmonary vascular and kidney in lupus mice models.
Keywords: Mesenchymal stem cell secretome- anti-dsDNA antibodies- Complement C3- histological features of pulmonary vascular- histological features of kidney- Lupus
