Hypoglycemic Activity of Combination of Moringa Leaves And Sorghum Bran Aqueous Extract in Streptozotocine-Induced Hyperglycemic Rats. EMMY NOVIARINI NINGTIYAS
Ministry of Agriculture of the Republic of Indonesia
Abstract
Diabetes Mellitus II (T2D) is a metabolic disorder characterized by insulin resistance and pancreatic β--cell dysfunction as a consequence of unsettled hyperglycemia. The increase in blood sugar levels will trigger the onset of oxidative stress condition characterized by an imbalance between the number of free radicals and antioxidants. A recent study done aqueous extract of moringa leaves and aqueous extract of sorghum bran effective of lowering blood sugar levels but the research is conducted in the form of a single and has never done a combination between both the extracts. This research was conducted to evaluated the antidiabetic activity and find out the effect of the combination of aqueous extract of moringa leaves and aqueous extract of sorghum bran against glucose levels in rats induced streptozotocin. Combination of aqueous extract of moringa leaves and aqueous extract of sorghum bran (50:50) showed total phenol of 28.60 mgGAE/g and antioxidant activity of IC50 of 112.67 ppm. The results of in vivo testing showed that the optimal dosage of combination aqueous extract of moringa leaves and aqueous extract of sorghum bran is 3,6 ml/200g bw. The antioxidant and antidiabetic activity improved pancreatic beta cells profile of diabetic rats and reduced blood glucose levels by 59.8% in the fourth week.
