ELECTROLYTES AND ANTIOXIDANTS PROFILE OF BLOOD SERUM HYPERTENSION RAT WITH ADMINISTRATION OF INDONESIAN PROPOLIS Ade Heri Mulyati(a*), Ahmad Sulaeman (b), Sri Anna Marliyati(b), Mohamad Rafi (c)
a)Department of Chemistry, Faculty of Mathematics and Natural Sciences, Pakuan University
Pakuan Street, Bogor City, West Java, Indonesia 16143
*adeheri.mulyati[at]unpak.ac.id
b) Department of Community Nutrition, Faculty of Human Ecology, IPB University,
Bogor 16680, Indonesia
c)Department of Chemistry, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Bogor 16680, Indonesia
Abstract
Propolis contains various bioactive compounds which can be used to prevent and treat hypertension. Decreasing blood pressure with bioactive compounds can occur through various metabolic pathways, increased potassium channel pathways, calcium channel blockers, angiotensin converting enzyme inhibitors, increased antioxidant levels, decreased oxidative stress and other pathways. This study aims to analyze the electrolyte and antioxidant profile in blood serum of hypertensive rats by administering the ethanol extract of Indonesian propolis. This research was conducted by analyzing the content of electrolyte compounds (sodium, calcium and magnesium) using ICP-OES, analyze of antioxidant levels (Nitric oxide, 8-isoprostane, superoxide dismutase (SOD) and malondialdehyde (MDA)) using the Enzyme-linked immunosorbent assay (ELISA). The results of this study found that Indonesian propolis increase levels of serum K+ in hypertensive rats significantly, especially propolis from Lampung and South Sulawesi. Serum Na+ levels were also found increasing after propolis administration for seven day, while Ca2+ levels did not differ significantly between groups. Although statistically not significantly different, Indonesian propolis tends to increase NO levels. In addition, serum SOD levels were significantly increased with the administration of Indonesian propolis. There was no decrease in serum levels of 8-isoprostane and MDA after the administration of Indonesian propolis.
Keywords: Indonesian propolis, bioactive compounds, antihypertensives, electrolytes, antioxidants
