IN-VITRO WOUND HEALING ACTIVITIES OF ESSENTIAL OIL OF WHITE TURMERIC (Curcuma zedoaria Rosc.) FROM LOWLAND AND HIGHLAND Suryati Syafri1,2, , Irwandi Jaswir1, Faridah Yusof3, Dachriyanus Hamidi2*
1 International Institute of Halal Research and Training, IIUM Malaysia
2 Faculty of Pharmacy, Universitas Andalas, Padang, West Sumatera, Indonesia
3Departement of Biotechnology Engineering, Kulliyah of Engineering, IIUM Malaysia
Email:dachriyanus[at]phar.unand.ac.id
Abstract
White turmeric (Curcuma zedoaria Rosc.) belongs to the Zingiberaceae family that produces essential oil. The essential oil contains chemical constituents, namely terpenoids, which have wound healing activity. This study aimed to compare the chemical profile of the essential oils of white turmeric in two different locations (lowland and highland) West Sumatera, Indonesia and observe their invitro wound healing activity. The chemical profiles of essential oils were determined using Gas Chromatography Mass Spectroscopy (GCMS) and Fourier Transform Infrared (FTIR). The highland and lowland EOs showed similar primary chemical components but vary in the quantity, which were Iso velleral (34.14%, 43.83%), Camphor (14.53%, 10.84%), Germacrone (6.85%,5.86%), isoborneol (5.41%,4.06%). The in-vitro wound healing activity was determined by proliferation and scratch assay. The result showed that both EOs enhanced fibroblast proliferation at concentrations of 0.1, 1, and \(10 \mu g/mL \) with more than 100%. The highland EOs showed higher proliferation activity compared to lowland EOs (p<0.05) in fibroblast proliferation but no significant difference with the positive control (curcumin).Then, scratch assay after 48 hrs incubation showed that highland EOs had higher wound closure of 90.86% at the concentration of \(0.1 \mu g/mL \). It can be concluded that the place of cultivation influenced chemical composition and biological activities.
Keywords: white turmeric, fibroblast proliferation, scratch assay, GC-MS, FT-IR spectroscopy
