Immobilization of crude polyphenol oxidase purple eggplant extract on chitosan membrane for removal of phenol waste water. Anceu Murniati1*, Buchari Buchari2, Suryo Gandasasmita2, Zeily Nurachman3, Shanti Shardi1, Irsyaad Fauzi R1, Arie Hardian1, Jasmansyah1, Hernandi Sujono1, Restu Muchammad Ibrahim1, Maulida Rahayu1
1Analytical Chemistry Research Group, Faculty of Sciences and Informatics
Universitas Jenderal Achmad Yani, Jl. Terusan Jenderal Sudirman, Cimahi, Indonesia
2Analytical Chemistry Research Group, Faculty of Mathematics and Natural Sciences,
Bandung Institute of Technology, Jl. Ganesha No. 10 Bandung 40132, Indonesia
3Biochemistry Research Group, Faculty of Mathematics and Natural Sciences,
Bandung Institute of Technology, Jl. Ganesha No. 10 Bandung 40132, Indonesia
Abstract
This research studied the synthesis of chitosan (CS) membrane with the basic component of polyphenol oxidase (PPO) from purple eggplant extract added with \(Cu^{2+}\) ions. The activity of the immobilized PPO in the CS-membrane was determined by the UV-visible spectrophotometer methods. The results showed that the membrane characteristics of CS-crude PPO-\(Cu^{2+}\) purple eggplant extract: diameter = 9 cm, thickness = 0.021 cm- immobilized PPO activity= 2894.99 U. The performance of the membrane is designed as a filtration with a dead-end system, with three (3) times the filtration process, and ten (10) times the use of a batch system membrane. The application of the CS-crude PPO-\(Cu^{2+}\) membrane was carried out on samples of artificial waste and textile waste solutions. The results achieved from the samples of artificial waste and textile waste, respectively, include the rejection = 68.77% and 63.69%- the conversion of phenol into catechol = 16.92% and 17.19% - the conversion of phenol to quinone = 42.96% and 42.64%- flow rate = \( \pm \)11 L/h and \( \pm \) 5 L/h- membrane flux= 10007.99 \( L/m^2.h \) at a pressure of 1 bar- permeability= 300-1000 \( L/m^2.h.bar \). The membrane life time is stable up to 8 times of use from 10 times of use.
