IMMOBILIZATION OF AIR FILTER MADE OF CHITOSAN STABILIZED SILVER AND ZINC OXIDE NANOPARTICLES AND THE ANTIBACTERIAL PROPERTIES Muhammad Iqbal Hidayat1, Muhammad Adlim*1,2, Ilham Maulana3, Suhartono Suhartono4, Zinatul Hayati5, Noor Hana Hanif Abu Bakar6
1Graduate School of Mathematics and Applied Science, Universitas Syiah Kuala, Darussalam Banda Aceh, 23111, Indonesia
2Chemistry Department, FKIP, Universitas Syiah Kuala, Darussalam Banda Aceh, 23111, Indonesia
3Chemistry Department, Faculty of Math and Natural Science, Universitas Syiah Kuala, Darussalam Banda Aceh, 23111, Indonesia
4dBiology Department, Faculty of Math and Natural Science, Universitas Syiah Kuala, Darussalam Banda Aceh, 23111, Indonesia
5eDepartment of Microbiology, School of Medicine, Universitas Syiah Kuala, Banda Aceh, Indonesia
6School of Chemical Science, University Sains Malaysia, 11800 USM, Penang, Malaysia
Abstract
An air-contaminated microorganism is being concerned indoors, especially during a disease outbreak- therefore, intensive study on antimicrobial air filters is growing. This study explores the preparation of stabilized and immobilized silver and zinc oxide nanoparticles and their antibacterial properties on simulated bacterial contaminated air. Chitosan stabilized silver nanoparticles (chi-Ag NPs) and chitosan stabilized zinc oxide nanoparticles (chi-ZnO NPs) have successfully prepared with the green chemistry method. Silver ion stabilized with chitosan, reduced with visible light irradiation, and immobilized onto silica gel beads. It has confirmed as nanoparticles in UV-Vis and TEM methods. Silica gel beads coated chi-Ag NPs (SiG chi-Ag NPs) prevented the growth of Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacteria in agar media. Silver was substituted with zinc oxide nanoparticles to minimize the processing costs. The immobilization of Zn ions and ZnO nanoparticles onto silica gel beads as compatible support has successfully studied and characterized. Silica gel beads coated chi-ZnO NPs (SiG chi-ZnO NPs) exhibited higher bacterial activities against Gram-positive and Gram-negative bacteria than SiG chi-Ag NPs.
