FORMULATION OF SALAM LEAF EXTRACT NANOEMULSION (Syzygium polyanthum) AS ACTIVE INGREDIENTS FOR THE PRODUCTION OF FACIAL ANTIOXIDANT SERUM A. Khoiruddin Yusuf (1), N. Fitri (2*), M. Idris (3) , S. Khoirul Himmi (4), N. Nurfajrin Solihat (5)
(1) Study Program of Chemistry, Department of Chemistry, Universitas Islam Indonesia
(2) Magister of Chemistry, Department of Chemistry, Universitas Islam Indonesia
(3) Stikes Rejakwesi Bojonegoro
(4) Research Center for Applied Zoology, National Research and Innovation Agency (BRIN)
(5) Research Center for Biomass and Bioproducts, National Research and Innovation Agency (BRIN)
Abstract
ABSTRACT
The aim of this research is to formulate a nanoemulsion of salam leaf extract as an active ingredient of antioxidant serum. The steps of this research include (1) extraction by maceration and Microwave-Assisted Extraction (MAE)- (2) Extract characterization- (3) formulation of nanoemulsion using the Self-Nanoemulsifying Drug Delivery System (SNEDDS) method- (4) Nanoemulsion testing: stability test, antioxidant activity test, and irritation test- and (5) nanoemulsion identification and characterization which includes LC-MS analysis, particle size, percentage transmittance, pH, and viscosity. The results showed that: (1) yields of the maceration method and MAE were 2.48% and 1.97% respectively- (2) antioxidant compounds in Salam leaf extract that were identified by LC-MS are Isorhamnethin, Icariin, Quercetin-3-O-di-hexose-O-pentoside, Proanthocyanidin tetramer, Procyanidin A-type trimer arabinoside, and Morusin isomer- The IC50 of the maceration and MAE extract were 17.1 ppm and 20.78 ppm- (3) Nanoemulsions were made in 3 formulas (0.1- 0.3- and 0.5 g) with fixed variables of capryol 90, tween 20, and PEG 400 (1.5- 2.5- and 1)- (4) stability test showed that only F1 was stable- the IC50 value of nanoemulsion is 950.3183 ppm- and the F1 irritation test showed no erythema and edema- (5) The particle size of F1 is 124.6 nm with a transmittance value of 96.6%, a pH of 5.45, and a viscosity of 79.07 cP.
