GREEN TEA EXTRACT (CAMELLIA SINENSIS L.) POTENTIAL TO LIVER OF HYPERURICEMIA RATS (RATTUS NORVEGICUS) FED WITH HIGH PURINE DIET Putranty Widha Nugraheni - Chanif Mahdi - Fitria Rahmawati - Rizki Nur Analita - Lalak Tarbiyatun Nasyin Maleiva
a) Department of Environmental Engineering, University of Tanjungpura
Jl. Prof. Dr. H. Hadari Nawawi, Kota Pontianak, Kalimantan Barat 78124 Indonesia
putranty[at]teknik.untan.ac.id
b) Department of Chemistry, University of Brawijaya
Jl. Veteran, Kota Malang 65145 Indonesia
chanif[at]ub.ac.id
c) Department of Chemistry Education, University of Lambung Mangkurat
Jl. Brigjen Jalan Hasan Basri, Kota Banjarmasin, Kalimantan Selatan 70123 Indonesia
rizki.analita[at]ulm.ac.id
d) Department of Chemistry Engineering, University of Tanjungpura
Jl. Prof. Dr. H. Hadari Nawawi, Kota Pontianak, Kalimantan Barat 78124 Indonesia
putranty[at]teknik.untan.ac.id
Abstract
Uric acid produced in tissues containing xanthine oxidases, like in the small intestine and the liver as purine metabolism of adenine and guanine. High uric acid levels can cause damage to cell membranes due to the lipid peroxidase chain reaction. Foods that contain high purines activate the xanthine oxidase enzyme causing free radicals to increase. Free radicals attack polyunsaturated fatty acids (PUFA) by forming carbon, peroxyl, and lipid peroxidases. PUFA will be broken into simple compounds such as pentane, ethane, and aldehydes, leading to malondialdehyde (MDA) formation. High MDA levels illustrate the process of cell membrane oxidation leading to cell membrane damage. Hyperuricemia is often treated using synthetic drugs such as allopurinol- however, it can cause side effects. Some people will choose medicinal plants with minimal side effects. Green tea (Camellia sinensis L.) contains many polyphenol antioxidants, especially flavonoids, also has a strong antioxidant effect by exerting multiple mechanisms such as inhibiting several enzymes, including antiphospholipid peroxidase, free radical scavengers, metal bonds, and xanthine oxidase (XOD). Statistical analysis results proved that green tea with a dose of 600 mg/kgBW can lower MDA levels of liver by 84.87% (p<0.01), decreased xanthine oxidase (XOD) activity by 35.36% (p<0.01), and improved liver histopathology.
