Chemotherapeutic Prospects of Dasycladaceae: Organic Extracts of Bornetella nitida from Selayar Island against HeLa Cell Lines Bahrun (a), Tatsufumi Okino (b), Firdaus (a), Herlina Rasyid (a), Nunuk Hariani Soekamto (a)
a) Department of Chemistry Hasanuddin University, Makassar, 90245, Indonesia
b) Faculty of Environmental Earth Science, Hokkaido University, Sapporo, 060-0810, Japan
Abstract
Cancer is the highest mortality disease caused by an unhealthy lifestyle. This research is the efforts to find natural product-based chemotherapeutic agents to solve this problem. Bornetella nitida is a Dasycladacean that is potential to be utilized due to the diversity of phytochemicals and the lack of exploration. The search begins with the phytochemical extraction using gradient maceration with n-hexane, chloroform, ethyl acetate, and methanol. Toxicity tests, antioxidant activity, cytotoxicity tests, phytochemical analysis, and molecular docking were also conducted. The B. nitida extracts were moderate toxicity, LC50 ranging from 116.06 to 713.12 ug.mL-1. The DPPH free radicals scavenging activity varied due to the solvent polarity, IC50 values 22.20, 87.77, 401.55, and 752.08 ug.mL-1 of n-hexane, chloroform, ethyl acetate, and methanol extract respectively. Meanwhile, cytotoxic against HeLa cell lines ranged from 281.09 to 1329.33 ug.mL-1, the highest activity shown by with ethyl acetate extract. GC-MS analysis showed the presence of octadec-9-enoic acid, phenol-2,4-bis(1,1-dimethylethyl), 9,12-octadecadienoic acid, 1,2-benzenedicarboxylic acid, 2,6,10-trimethyl-14-ethylene-14-pentadecene, hexadecatrienoic acid, and phytol. The compounds showed good interaction against protein target (binding energy < -5 kcal.mol-1). The results of this study indicate that the B. nitida extract has the potential to be developed as a chemotherapeutic agent.
Keywords: Marine natural product- bioactive- chemotherapy- green algae
