NANOPARTICLES EXTRACT ROSELLA FLOWER PETAL (Hibiscus sabdariffa L.) ACTIVITY TEST AS ANTIFUNGAL AGAINST THE GROWTH OF Candida sp AND Tricophyton sp BY IN VITRO Budi Saputra, Randi Kristiana, Retno Diyah Utari, Jasmansyah , Valentina Adimurti K, Tiani Yunita, Tiana Yunita, Siti Rosita
Universitas Jenderal Achmad Yani
Abstract
Has been developed the technology for making nanoparticles from rosella flower petal extract (Hibiscus sabdariffa L.) of activity test as antifungal toward the growth of candida albicans and Tricophyton mentagrophytes, This the study aims to etermine the secondary metabolite content of rosella flower petal extract, to determine the activity as antifungal its effective concentration in inhibiting toward the growth of C. albicans and T. mentagrophytes. Rosella flower fetal extract was obtained by maceration by using 70% of ethanol dissolver and the extract obtained was carried out by phytochemical screening. Manufacture of nanoparticles obtained by ionic gelation method using chitosan. The antifungal activity was tested using the disc diffusion method with concentrations of 10%, 20% and 40%. The positive control used was nistatin (100 ppm) and the negative control used 2% DMSO. The results of this study indicate that rosella flower petal extract contains flavonoid compounds, saponins, quinones and phenols. Rosella flower petal nanoparticles extract concentrations of 10%, 20% and 40% can provide activity that inhibits the growth of the test fungus. There was an increase in the diameter of the inhibition zone at each increase in concentration, namely 10% (7 mm), 20% (7.7 mm) and 40% (10.5 mm) against fungus C. albicans and concentrations of 10% (7 mm), 20% (8.7mm) and 40% (10.2 mm) against the fungus T. mentagrophytes. All variations of extract concentration seed showed antifungal activity. The effective concentration is concentration 40%, it showed that the activity level was strong antifungal activity in inhibiting the growth of C. albicans and T. mentagrophytes.
