Rapid Secondary Metabolites Profiling of Underutilized Legumes Using Fourier Transformation Infrared (FT-IR) Based Metabolomics Amelinda Pratiwi*1, Siti Aisyah1
Department of Chemistry, Universitas Pendidikan Indonesia
Abstract
Indonesia has several legumes species of which some of them has not widely explored, including cowpea (Vigna unguiculata), red bean (Phaselus vulgaris), and rice bean (Vigna umbellata). This study aims to differentiate the metabolite profiles. The metabolite profiling analysis of three types of legumes was carried out using FTIR-based metabolomics. The legumes powder was immediately dissolved in KBr and analyzed using FTIR spectroscopy. The analysis data multivariate of FTIR spectrum was then carried out by UnscramblerX. The metabolite profiles of the three legumes could be distinguished through PCA analysis. Based on the loading plot analysis, there were 4 ranges of wave numbers contributed to the different profiles of each bean, namely in the fingerprint area to 2000, 2200 - 2500, 2700 - 3200, and 3500 - 4000 cm-1. The different absorption for each type of bean was suggested to be the absorption of the -C-H band from carbohydrates and proteins, or amides for rice bean and structural carbohydrates for cowpea. The results of the hierarchical complete linkage (HCA) analysis of the FTIR spectrum of cowpea, rice bean, and red bean showed that red bean had the most distant relationship compared to the other two beans, while rice bean and cowpea had close similarities.
