OPTIMIZATION CONCENTRATION OF ETIL ASETAT FRACTION Orthosiphon aristatus TO ANTIBACTERIAL ACTIVITY Propionibacterium acnes IN VITRO Ike Widyaningrum, Dian Novita Wulandari, Nuri Amalya, Putri Nabila Khalisa
Universitas Islam Malang
Abstract
Propionibacterium acnes (P. acnes) is one of the bacteria that causes acne that requires various alternatives, one of which is antibiotics. Ethyl acetate is known to be a semipolar solvent. Nowadays, cosmetic preparations using herbs are starting to develop. In this study, the Orthosiphon aristatus plant fractionated with ethyl acetate was used. The concentrations used were 100,000 ppm, 75,000 ppm, 50,000 ppm, and 10,000 ppm. The antibacterial activity testing method used is ZOI (Zone of inhibition) by observing the clear zone. Based on phytochemical tests, it is known that the ethyl acetate fractionation of Orthosiphon aristatus leaves contains flavonoids, alkaloids, and terpeneoids. The results of the research obtained are known that the increasing concentration of the ethyl acetate fraction of Orthosiphon aristatus, it is known that the clear zone produced is also increasing.
