Efficacy Antibacterial Activity of ZnO Nanoparticles Result Green Synthesis using Soursoup Leaves (Annona muricata L.) against Cutibacterium acnes Aisy Hanifah1, a), Siti Imroatul Maslikah1, b), Sri Rahayu Lestari1, and Awil Endar Pramesti1
1Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, Jl. Semarang 5, Malang 65145, Indonesia
Acne vulgaris (AV) is a global health issue of skin infection diseases, can be caused by bacteria Cutibacterium acnes. AV treatment using topical or oral antibiotics has side effects and causes bacterial resistance. ZnO nanoparticles have potential as antibacterial agents. Generally, nanoparticles synthesized through physical and chemical methods. However, both methods have disadvantages that producing toxic residues and high operating costs. This research used green synthesis method (environmental friendly), using soursop leaf extract (Annona muricata). Soursop leaf extract contains secondary metabolites like flavonoids, tannins, alkaloids, and terpenoids have the potential as reducing and capping agents to prevent agglomeration of nanoparticles. The aim of study to determine the potential and effective concentration of ZnO nanoparticles as antibacterial against Cutibacterium acnes. Experimental research based on completely randomized design with 4 treatments: 32 mg/ml, 64 mg/ml, 128 mg/ml, 256 mg/ml. Data analysis through one way ANOVA test (p<0.05), followed by Duncan test (p<0.01). The results showed that ZnO nanoparticles had antibacterial activity, there were significant differences between treatments, concentration of 32 mg/ml has minimum antibacterial effect with diameter of inhibition zone 16.6 mm, and the most effective concentration was 256 mg/ml with diameter of inhibition zone 22.5 mm.
