ENZYMATIC DEGRADATION OF OIL PALM EMPTY FRUIT BUNCH USING LACCASE FROM Trametes hirsuta EDN 082 Fitria Maulita (a), Anja Meryandini (a), Dede Heri Yuli Yanto (b*), Sri Listiyowati (a), Maulida Oktaviani (b), Oktan Dwi Nurhayat (b)
a) IPB University, Bogor, Indonesia
b) Research Center for Applied Microbiology, National Research and Innovation Agency (BRIN), Cibinong Bogor, Indonesia
*dede.heri.yuli.yanto[at]brin.go.id
Abstract
Enzymatic degradation of lignocellulosic biomass of oil palm empty fruit bunches (OPEFB) by laccase of white rot fungus Trametes hirsuta EDN 082, has good potential in bioethanol production. This study aimed to analyze changes in the structure of lignin resulting from enzymatic degradation by laccase extract from T. hirsuta EDN 082. Enzymes are produced by solid-state fermentation (SSF). OPEFB lignin was then enzymatically hydrolyzed in a shaker incubator for 24 hours using crude laccase extract from T. hirsuta EDN 082. Chemical structure analysis was carried out using Py-GCMS, which detected lignin degradation products, namely phenol, creosol, syringalacetone, isoeugenol, benzaldehyde, and guaiacyl alcohol. Fourier transform infrared (FTIR) showed a change in the functional group band at 1161-1645 cm-1.
Keywords: Laccase- White rot fungus- Lignocellulosic biomass
