Bacterial endophytes Achromobacter xylosoxidans growth and quinoline secondary metabolite production in modified C:N ratio sugar cane juice Siti Zubaidah, Rahmi Masita, and Fauzi AKhbar Anugrah
Department of Biology, faculty Mathematics and Natural Sciences, Universitas Negeri Malang, Malang, Indonesia
Abstract
Endophytic bacteria Achromobacter xylosoxidans in previous studies were known to produce metabolites similar to their host plants on agar media. In this study, sugarcane juice was used as the medium by modifying the ratio of carbon and nitrogen (C/N) as an alternative for producing secondary metabolites of endophytic bacteria. The results of the analysis of the water content of sugarcane juice showed that the total carbon and nitrogen were 1.08% and 0.003%, respectively. Bacteria were grown on a modified medium by adding urea so that the C/N ratio of the media became 3:1, 5:1, 7:1, 9:1, and 11:1. In addition to different C/N ratios, the optimization medium used the same initial pH of the medium was 5,6, 7, 8, and 9, and growing at three different temperatures 23˚-C, 30˚-C, and 37˚-C. The growth medium and the optimum growth conditions for A. xylosoxidans were 9:1 C/N ratio, pH 7, and the optimum temperature was 30˚-C with biomass of 1.09 X 108 CFU/ml. Moreover, based on the results of the qualitative Thallequin test, it is concluded that the bacteria A. xylosoxidans still has the potential to produce quinine and quinidine metabolites in the treatment of sugarcane juice medium urea.
