The Production of Urease by New Isolated Gram Negative Bacteria Using Different Substrates Frida Ayu Wahyuwati, Danu Risqi Yudiargo, Fellycita Kartika Afriliona, Fida Alya Putri Rimansa, Izzatul Islami, Karimatul Millah, Nadhifah Astari Putri, Endry Nugroho Prasetyo*
Gedung H, Department of Biology, Faculty of Science and Data Analytics, Institut Teknologi Sepuluh Nopember, Kampus ITS, Keputih, Sukolilo, Surabaya 60111, Indonesia
Abstract
Urease is an enzyme that contains nickel which produced by ureolytic microorganism. Urease catalyzes the hydrolysis of urea to carbon dioxide and ammonia. Microbial urease has wide applications in biotechnology, construction, and geoenvironmental engineering field. Several organic urea based substrates from agricultural waste are often used as urease production media. The aim of this study was to determine the effect of various substrate for urease production. Two isolates of Gram negative bacteria were selected for urease fermentation while measuring its qualitative activity. The result showed that the highest urease activity was achieved with addition of urine as a substrate compared to others.
