The Clonal Spread of Methicillin-Resistant Staphylococcus Aureus (MRSA) Strains by Antibiogram Finger-Printing from Discharge Patiens in Surgical Wards and Medical Wards Dr. Soetomo Hospital, Surabaya, Indonesia Aima Insana1a), Kuntaman Kuntaman2, Marijam Purwanta2, Mardiana Lelitawati3,4
1. Medical Laboratory Technology, Poltekkes Kemenkes Banjarmasin, Indonesia
2. Department of Microbiology, Faculty of Medicine, Universitas Airlangga/Dr. Soetomo Hospital Surabaya, Indonesia
3. Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, Jl. Semarang 5, Malang 65145, Indonesia
Abstract
MRSA is a major pathogen associated infections acquired in the hospitals cause increased morbidity and mortality. The aim of this study was to analyze clonal spread of MRSA strains from discharge patiens in Dr.Soetomo Hospital based on antibiogram finger-printing. The samples were 49 isolates patients on discharge in surgical and medical wards colonized with MRSA in nose and throat. Isolates were taken from stock culture of MRSA collected from nose and throat in previous studies. The antibiogram test was carried out using 12 antibiotics by diffusion agar method in Muller Hinton Agar. Antibiotic resistant pattern of MRSA were used to classify antibiogram type. Isolates were considered to belong to different antibiotypes if at least one or more difference was observed. Antibiogram analysis of 49 isolates showed 18 types. Type 24, the predomimant resistance pattern was ciprofloxacin, cefoxitin, gentamicin, penicillin and tetracycline occured in 17 isolates (34,7%) consisted of 10 isolates (59%) from surgical wards and 7 isolates (41%) from medical wards. It showed high spread of MRSA between patiens in Dr. Soetomo Hospital, Surabaya. Clonal spread analisys in surgical and medical wards showed no significant difference, MRSA nose usapan (p = 0,642) throat usapan (p = 0,698).
