Microbial Urease Extracted from Urban Sewer Bacteria Responds to Different Temperature Karimatul Millah, Danu Risqi Yudiargo, Fellycita Kartika Afriliona, Fida Alya Putri Rimansa, Frida Ayu Wahyuwati, Izzatul Islami, Nadhifah Astari Putri, Endry Nugroho Prasetyo*
Gedung H, Departement of Biology, Faculty of Science and Data Analytics, Institut Teknologi Sepuluh Nopember, Kampus ITS, Keputih, Sukolilo, Surabaya 60111, Indonesia
*endry[at]bio.its.ac.id
Abstract
Urease is an enzyme produced by ureolytic microorganisms that hydrolyzes urea to ammonia and carbamates. Microbial urease is applied in medicine, biotechnology, agriculture, and construction. In the construction industry, urease plays a key role in the precipitation of calcium carbonate which depends on the rate of urea hydrolysis. The rate of urea hydrolysis is mainly influenced by temperature, pH, and substrate concentration. This study aims to determine the effect of temperature on the activity of urease produced by bacteria isolated from urban sewer. The urease activity was tested using the chromogenic method and the temperatures were set at 20, 40, and 60 degrees Celsius. The results of this study indicated that temperature affects urease activity up to 60 degrees Celcius which decreases the enzyme activity.
Keywords: Activity- Bacteria- Urban sewer- Urease- Temperature
