Sensitivity of Bulb Tissue for Detection of Fusarium Causes Moler disease In Shallot Salim Widono (1), Hadiwiyono (2), SH Poromarto (2), Supyani (2) and Nur Wahyuni (3)
(1) Student of Doctoral Program at Universitas Sebelas Maret (UNS) Surakarta
(2) Doctoral Program in Agricultural Sciences at Universitas Sebelas Mret (UNS) Surakarta
(3) Student of Agrotechnology Study Program at Universitas Sebelas Maret (UNS) Surakarta
Abstract
Moler caused by Fusarium oxysporum f.sp.cepae is a seed-borne disease, so the disease intensity in the field is depend on the amount of initial inoculum in the infected bulbs as seed. Therefore, the detection of seed-borne pathogens is important to determine the potential of their transmission. The aim of this research was to evaluate the sensitivity of the bulb tissue of shallot based on the pathogen Infection Index by incubation method on agar media. The experiment was conducted using a completely randomized design with five replications and three varieties of shallots (Thailand, Bima, and Bauji). Each treatment unit was placed in a Petri dish with a diameter of 20 cm, containing 40 mL PDA on which 16 pieces of tissue at the tip, middle, and base of the bulb tissues were placed, which were incubated for 7 days. Pathogen Infection Index is a parameter that was observed macroscopically and microscopically. The results showed that the base of the bulbs tissue of the three varieties tested showed higher sensitivity than the other part to detect the presence of F. oxysporum f.sp. cepae in shallot.
