Evaluation of Apple Rose Leaf [Eugenia aqueum (Burn F.) Alston] Activity on Bacteria that Cause Dental Caries
Suwendar. Lisnur Wachidah, Yani Krisnamurti, Dini Aprilliani, Rifa Nabila Ruswandi, Annisa Ajeung Wulandari, Dieni Mardliyani, Nisa Fitriani

Universitas Islam Bandung
Jalan Rangga Gading no. 8
Bandung

Abstract

Dental caries is a health problem in the oral cavity with a high prevalence rate. One of the causes of dental caries is Streptococcus mutans. Apple rose leaves [Eugena aqueum (Burn F. Alston)] have been known to have antibacterial activity. The purpose of this study was to evaluate the activity of apple rose leaves against Streptococcus mutans. Evaluation of antibacterial activity was carried out in vitro using the agar diffusion test method by means of wells. The results showed that the ethanol extract and the extract fraction (n-hexane, ethyl acetate and water) of apple rose leaves had antibacterial activity on S. mutans. Apple rose leaf ethanol extract showed the strongest activity because it had the lowest minimum inhibitory concentration (MIC) compared to the three extract fractions, namely 0.78%, with an average inhibition zone diameter of 1.13 cm based on three observations. The MIC value of the n-hexane fraction was 3.13% with an average inhibition zone diameter of 1.07 cm, while the MIC values for ethyl acetate and water fraction were 1.56% each with an average inhibition zone diameter were 1.09 and 1.08 cm, respectively. Based on the results of these tests, it can be concluded that apple rose leaves have the potential to be developed into a natural toothpaste preparation that is efficacious in preventing dental caries.

Keywords: apple rose leaf, dental caries, Streptococcus mutans

Topic: Material Science