Effect of Germination and Elicitation using Tempeh Fungus on The Phenolic Profile of Jack Bean (Canavalia ensiformis L.)
Siti Aisyah1*, Amelinda Pratiwi1, Rita Chintia Dewi1, F.M Titin Supriyanti1

1Department of Chemistry, Universitas Pendidikan Indonesia

Abstract

Phytoalexin is an antimicrobe compound synthesized in the plant as a result of pathogen response and chemical interaction. Elicitation is one of the induction methods to form a phytoalexin compound. The aim of this research is to observe the effect of the combination of germination and elicitation using tempeh fungus on the isoflavonoid profile of jack bean. The jack bean was germinated for two to seven days following five days of elicitation using tempeh fungus. The dried powder of jack bean was extracted using methanol 80% (v/v). The isoflavonoid profiles were analyzed using LC-MS/MS. The results show that germination and combination germination-elicitation can change the isoflavonoid profile. It showed that 2^-hydroxygenistein and medicarpin can be elicited in jack bean by using tempeh fungus.

Keywords: Germination, Elicitation, Isoflavonoids, Phytoalexin, LC-MS/MS

Topic: Food Chemistry