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ANTIGOUT ACTIVITY OF THE Spatholobus littoralis Hassk. FRACTION AGAINST XANTHINE OXIDASE: INHIBITION KINETICS AND ITS METABOLITE PROFILE (a) Department of Chemistry, Faculty of Mathematics and Natural Sciences, IPB University, Abstract The Spatholobus littoralis Hassk. (S. littoralis Hassk.), also referred to as the bajakah tampala is a native plant in Indonesia and has been widely used in traditional Dayaknese medicine for non communicable degenerative diseases. One of these illnesses, known as gout, is caused by an excessive amount of uric acid in the blood, which is the catalytic byproduct of a xanthine oxidase (XO) enzyme. In this work, we investigated in vitro inhibition kinetics of XO and putatively identified bioactive compounds from the stem of bajakah tampala using LC MS MS. Fractionation was performed to obtain n-hexane, dichloromethane, and 1-butanol fractions from water and 70% ethanol extract. 1-Butanol fraction of 70% ethanol and the water extracts displayed potent inhibitors of XO with IC50 116.91 and 137.15 mg/L, respectively. Furthermore, Lineweaver-Burk plot analysis showed that the 1-butanol fraction from the two extracts inhibited XO competitively. The fraction from these two extracts has been further identified as a bioactive compound. The three highest peaks from 1-butanol fraction of 70% ethanol extract are formononetin, piscidic acid, and (15Z) 9,12,13, Trihydroxy 15 octadecenoic acid- whereas from 1-butanol fraction of water extract are piscidic acid, methylmalonic acid, and DL malic acid. Based on the composition of the compounds, the phenolics dominated in 1-butanol fraction of 70% ethanol extract by 58.97% and 52.17% for 1-butanol fraction of water extract. These findings revealed that 1-butanol fraction from the two extracts is promising as an antigout treatment in the future. Keywords: Gout, Lineweaver-Burk Plot, LC-MS/MS, Spatholobus littoralis Hassk., Xanthine Oxidase Topic: Natural Products and Biological Chemistry |
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