THE INHIBITION ACTIVITY OF Aglaia elaeagnoidea STEM BARK EXTRACT ON THE FORMATION OF CALCIUM OXALATE CRYSTALS
Asep Supriadin a), Ahmad Saepul Fikri a) and Frisca Damayanti b)

a) Chemistry Department, Faculty of Science and Technology, UIN Sunan Gunung Djati
b) Research Center for Plant Conservation and Botanical Gardens - BRIN

Abstract

Calcium oxalate is the main component in kidney stones that are formed due to the occurrence of supersaturation in the urine. Medical and traditional methods of treatment and prevention of kidney stone disease still do not provide effective results, so that efforts to find plants that have the potential to prevent kidney stone formation continue to be carried out. Aglaia elaeagnoidea plants, especially the stems, are known to have high phenolic content where phenolic compounds have been shown to inhibit the formation of calcium oxalate crystals. Therefore, the purpose of this study was to analyze the total phenolic content and to examine the inhibitory activity of Aglaia elaeagnoidea bark extract on the formation of calcium oxalate crystals. The process of analysis and testing begins with extracting the bark powder of Aglaia elaeagnoidea using n-hexane, ethyl acetate, and methanol using the multilevel maceration method. Analysis of total phenolic content was carried out by colorimetric method using Folin-Ciocalteu reagent and gallic acid as phenolic standard measured using UV-Vis spectrophotometer. There were obtained phenolic content of n-hexane, ethyl acetate and methanol extracts 6.75- 14.89- and 104.97 mg GAE/g extract. The results of the inhibitory activity of the bark extract of Aglaia elaeagnoidea on the formation of calcium oxalate crystals showed that the methanol extract under glucose environmental conditions had the highest activity with an IC50 value of 203.17 mg/L. The process of inhibition of the formation of calcium oxalate crystals occurs in the presence of the active substance which is adsorbed on the crystal surface and forms a single layer that interacts with physisorption which occurs spontaneously. The inhibitory activity of calcium oxalate crystal formation was influenced by the phenolic content in the bark extract of Aglaia elaeagnoidea, with increasing phenolic content in the extract, the inhibitory activity that occurred also increased.

Keywords: Aglaia elaeagnoidea- inhibitory activity- total phenolic content- turbidity- calcium oxalate crystals

Topic: Natural Products and Biological Chemistry