Metabolite Fingerprinting of Botanical Parts of Erythrina Crista-Galli Using Pyrolysis-Gas Chromatography/Mass Spectrometry
Abd. Wahid Rizaldi Akili (a), Ari Hardianto (a), Maya Ismayanti (b), Tati Herlina (a*)

(a)Department of Chemistry, Faculty of Mathematics and Natural Science, University Padjadjaran, Jatinangor, West Java 45363, Indonesia
*tati.herlina[at]unpad.ac.id

(b)Research Center for Biomaterials, Indonesian Institute for Sciences (LIPI), Jl. Raya Bogor KM 46 Cibinong Bogor, West Java, Indonesia

Abstract

Erythrina crista-galli is commonly used in folk medicines for its pharmacological properties which are associated with the metabolite constituents. The botanical parts of E. crista-galli have various bioactivity such as anti-inflamatory, antimicrobial, antimutagenic, etc. The objective of this study is to uncover the similarity and clustering of botanical parts of E. crista-galli based on their chemical content by means of metabolite fingerprinting. The botanical parts of E. crista-galli, including bark, flower, leaves, roots, and twigs, were subjected to pyrolysis-gas chromatography/mass spectrometry. The samples were pyrolyzed using a multi-shot pyrolyzer. The relative abundance of the pyrolysate was used for multivariate analysis. Principal component analysis (PCA) allows to characterize the roots of E. crista-galli by the highest relative abundance of lignin G, followed by the twigs, bark, leaves, while the flower has the least relative abundance of lignin G. Hierarchical cluster analysis allows to cluster the botanical parts of E. crista-galli into three different clusters based on their chemical component similarity, i.e. flower and leaves, twigs, roots, and bark.

Keywords: Erythrina crista-galli, Pyrolysis-GC/MS, multivariate analysis, principal component analysis, hierarchical clustering analysis.

Topic: Natural Products and Biological Chemistry