Textile Wastewater Treatment using Polypyrrole Membranes with the basic component of Crude Polyphenol Oxidase Extract from White Oyster Mushroom
Anceu Murniati1*, Buchari2, Suryo Gandasasmita2, Zeily Nurachman3, Arie Hardian1, Hernandi Sujono1, N A Fajriana1, G A Nugraha1 and Restu Muchammad Ibrahim1

1Department of Chemistry, Faculty of Sciences and Informatics, Universitas Jenderal Achmad Yani, Cimahi, Jl. Terusan Jenderal sudirman, Cimahi, Indonesia
2Analytical Chemistry Research Group, Faculty of Mathematics and Natural Sciences, Bandung Institute of Technology, Jl. Ganesha No. 10 Bandung 40132, Indonesia
3Biochemistry Research Group, Faculty of Mathematics and Natural Sciences, Bandung Institute of Technology, Jl. Ganesha No. 10 Bandung 40132, Indonesia

Abstract

In this research, polypyrrole (PPy) membranes have been synthesized with polyphenol oxidase (PPO) as the base materials isolated from white oyster mushroom (Pleurotus ostreatus), which plays an active role in the processing of textile wastewater. The amount of PPO immobilized in PPy was 28.49%, and PPO activity in white oyster mushrooms (4160 U- phenol 0.35 mM). PPy membranes were synthesized by electropolymerization of Pyrrole (Py) with the electrodeposition method (the composition of 0.05 M Py and crude PPO white oyster mushroom extract of 35% (v/v) in citrate buffer pH = 7). The electrolysis was carried out with a potential = 6 V- current = 1.063-1.142 A- the amount of immobilized PPO in the PPy matrix = 16.57 - 48.97 %, and the PPO activity of 1,640 - 4,160 U- 0.35 mM phenol as a substrate. Membrane application to textile wastewater with discontinuous flow rate obtained (decrease in pH = 14.86%- phenol = 6.80%- COD = 10.80%- BOD and TSS = 2:00% = 20.65%) and the use of membranes with a continuous flow rate (decrease in pH = 23.97%- phenol = 26.09%- COD = 11.54%- BOD = 2.46%- and TSS = 65.65%). The physical performance of membrane application to textile wastewater was studied with discontinuous flow rates obtained (flow rate = 48.19 - 51.50 L/h, flux = 3191.6 - 3387.5 L.m-2.h-1 and permeability = 319.2 - 338.7) and a continuous flow rate was obtained (flow rate = 48.19 L/h, flux = 3191.6 Lm-2.h-1 and permeability = 319.2 Lm-2.h-1.bar). Scanning Electron Microscopy (SEM) image shows the morphology of the membrane film of PPy/PPO white oyster mushroom extract that has been studied in the use of membranes before and after application of textile wastewater.

Keywords: white oyster mushroom, membrane, PPy/PPO, textile wastewater

Topic: Environmental Chemistry and Engineering