Metabolite Profiling of Sidaguri (Sida rhombifolia) With Different Drying Method and Xanthine Oxydase Inhibitory Evaluation
Dea Silviani (a*), Mohamad Rafi (a, b, c), Utami Dyah Syafitri (d), Wulan Tri Wahyuni (a, c)

a) Department of Chemistry, Faculty of Mathematics and Natural Sciences, IPB University, Jl. Tanjung Kampus IPB Dramaga, Bogor 16680, Indonesia
*deasilviani[at]apps.ipb.ac.id
b) Advanced Research Laboratory-Institute of Research and Community Service, IPB University, Jalan Palem Raya, Kampus IPB Dramaga, Bogor 16680, Indonesia
c) Tropical Biopharmaca Research Center, Institute of Research and Community Services, IPB University, Jl. Taman Kencana No. 3, Bogor 16128, Indonesia
d) Department of Statistics, Faculty of Mathematics and Natural Sciences, IPB University, Jl. Meranti kampus IPB Dramaga, Bogor, 16680, Indonesia

Abstract

Sidaguri (Sida rhombifolia) is a type of plant that has been widely used as traditional medicine because of its bioactive metabolites. The composition of metabolites in plants can be influenced by several factors, one of them is the drying process. This study aims to profiling metabolites in sidaguri with different drying methods and evaluate the xanthine oxidase inhibitory activity. The different drying methods used are oven drying (OD), sun drying (SD), and air drying (AD) by keeping the moisture content below 10%. LC-MS/MS and FTIR were used to determine the metabolites profile of sidaguri based on untargeted metabolomic approach. Grouping of sidaguri based on different drying methods were done by using principal component analysis (PCA). The results of metabolite profiling using LC-MS/MS showed that 24 metabolites in the sidaguri extract with different drying methods. 15 metabolites were found in the OD extract, 16 in the SD extract, and 20 in the AD extract. The peak area of each confirmed metabolite was then classified using PCA. In the other side, absorbance at wave numbers 1500-800 cm-1 from FTIR spectra were used for grouping using PCA. It is known that the sidaguri extracts were separated based on their respective drying methods with the total variance that could be explained were 95% (LC-MS/MS) and 99% (FTIR). Evaluation of xanthine oxidase inhibition showed that sidaguri could inhibit the performance of the xanthine oxidase enzyme in converting xanthine to uric acid by as much 35.97% (OD), 45.93% (SD), and 22.84% (AD) at concentration 50 ppm.

Keywords: Metabolomics- Drying method- Sidaguri- Xanthine oxydase

Topic: Analytical Chemistry