Kaolinite-Based Silica Coarse (SC) for Nucleic Acids Extractions Method
Marchia Marthalena Marintan (a), Fithriyah (c) , Yuni Krisnandi (b)

(a) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Indonesia, Depok, 16424, Indonesia.
(b) Solid Inorganic Framework Laboratory, Department of Chemistry, Faculty of Mathematics and Natural Sciences. Universitas Indonesia, Depok, 15424, Indonesia.
(c) Department of Microbiology, Faculty of Medicine, Universitas Indonesia, 10320 Jakarta, Indonesia.

Abstract

One of the strategies to overcome the COVID-19 disease is through RT-PCR test. The RT-PCR test is initiated by extraction of nucleic acids which requires silica column. Silica Coarse (SC) in the form of suspension, powder and discs columns- were prepared from kaolinite as an alternative extraction column to binding Ribonucleic acids (RNA). The RNA binding ability in SC was enhanced with chaotropic ions, embedded on the kaolinite framework. SC, then were studied its characterizations using FTIR, XRD, XRF, SEM, and BET measurement. This work shows that SC suspension column could extract RNA that amplified better in RT-PCR test than SC powder and discs columns.

Keywords: Silica Coarse, Chaotropic Ions, Kaolinite, Nucleic acid extraction, RT-PCR test

Topic: Inorganic Chemistry