Formulation and In-vitro Characterization of Bacterial cellulose-Nanoparticles Loaded with Mg(OH)2 and Ag
Henry F. Aritonang*, Shintya Maryani, Gillby Laseduw, Audy D. Wuntu

Physical Chemistry Division, Faculty of Mathematics and Natural Sciences, Sam Ratulangi University

Abstract

Bacterial cellulose (BC) is a type of natural cellulose widely synthesized by Acetobacter xylinum. However, BC itself does not have antibacterial properties that are important for any particular purpose. Therefore, some researchers insert materials that are antibacterial in BC. The objectives of this study were to develop bacterial cellulose (BC)-nanoparticles (NPs) loaded with Mg(OH)2 and silver (Ag) and to determine the composition of magnesium nitrate hexahydrate (MN) and silver nitrate (SN) precursors showing the best antibacterial properties. SNs of different contents (1, 3, 5, 7, and 9% of the mass of MN) were inserted with MN (5 g) into BC. This synthesis used the co-precipitation method by immersing BC into the two precursor solutions in an alkaline environment. The deposition of Mg(OH)2 and Ag NPs in the BC fibers was confirmed by a change in the color of the BC membrane from white to brownish. The profiles of Mg(OH)2 and Ag NPs deposited on BC fibers were relatively different. Mg(OH)2 particles tended to agglomerate and cover the fiber, while Ag particles also agglomerate but not as much as Mg(OH)2 particles. The composition of 9% SN produced BC/Mg(OH)2/Ag-9% material that had higher Mg(OH)2 and Ag NPs content and smaller size. In addition, BC/Mg(OH)2/Ag-9% was better at inhibiting the growth of E.coli and S.aureus.

Keywords: Formulation- Bacterial cellulose, Nanoparticle- Mg(OH)2- Ag- Antimicrobial

Topic: Polymer Chemistry & Bio-based Materials