Synthesis and Characterization of Chitosan-Polysulfone/PPO Membranes from Purple Eggplant Extract (Solanum Melongena L.)
Anceu Murniati1, Qina Ramdanyani1, Buchari Buchari2, Suryo Gandasasmita2, Zeily Nurachman3, Arie Hardian1

1Analytical Chemistry Research Group, Faculty of Sciences and Informatics
Universitas Jenderal Achmad Yani, Jl. Terusan Jenderal Sudirman, Cimahi, Indonesia
2Analytical Chemistry Research Group, Faculty of Mathematics and Natural Sciences,
Bandung Institute of Technology, Jl. Ganesha No. 10 Bandung 40132, Indonesia
3Biochemistry Research Group, Faculty of Mathematics and Natural Sciences,
Bandung Institute of Technology, Jl. Ganesha No. 10 Bandung 40132, Indonesia

Abstract

Membrane separation technology is still being developed to obtain optimal membrane performance in the separation process. Many uses membranes made of polymers using chitosan, but the brittle and hydrophobic nature of chitosan makes the filtration process less than optimal. Thus, it is necessary to add polymeric materials such as polysulfone which has the good mechanical quality and chemical stability and also has relatively large pores. The purpose of this research is a membrane with the basic ingredients of polysulfone, chitosan, and polyphenol oxidase (PPO) from purple eggplant (Solanum melongena L.) modified by Cu(II). This research was initiated by making a polysulfone-chitosan membrane, then carried out the PPO-Cu(II) immobilization method on a polysulfone-chitosan membrane with the composition of polysulfone/chitosan being A (55%:45%) and B (32%:68%) respectively. The results of the characterization of polysulfone-chitosan-A membranes thickness (0.005 plus minus 0.002 cm) and polysulfone-chitosan-B (0.0095 plus minus 0.001 cm)- the mass of polysulfone-chitosan-A membrane (0.036 plus minus 0.0015) g and polysulfone-chitosan-B membrane (0.087 plus minus 0.002 gram). PPO activity was immobilized on the polysulfone-chitosan-A membrane (56.92%) or 2934.65 U and polysulfone-chitosan-B membrane (64.08%) or 3608.82 U, with 8 mM catechol as substrate. Meanwhile, the degree of swelling on polysulfone-chitosan-A membranes (28.792%) and polysulfone-chitosan-B membranes (29.92%). The SEM image on the membrane can be observed as a microfiltration membrane with a pore size (1.5-6.12 micrometer).

Keywords: membrane, chitosan, polysulfone, PPO

Topic: Environmental Chemistry and Engineering