ISOLATION AND IDENTIFICATION OF LACTIC ACID BACTERIA FROM CHINESE CABBAGE
Assyifa Junitasari, Tina Dewi Rosahdi, Yuni Siti Lestari

Departement Chemistry, Faculty of Science and Technology, UIN Sunan Gunung Djati Bandung, Indonesia

Abstract

Waste is material that is disposed of from sources of human and natural activities that do not yet have economic value. Physically, vegetable waste is perishable due to its high water content, especially Chinese Cabbage waste. Lactic Acid Bacteria (LAB) are facultative anaerobic bacteria that can live in various of habitats. However, the availability is still low and the price is expensive. Therefore, it is necessary to isolate LAB from new sources such as Chinese Cabbage which has a high LAB content. The purpose of this study was the isolation and identification of lactic acid bacteria from Chinese Cabbage waste. Isolation was carried out to obtain isolates of lactic acid bacteria from Chinese Cabbage waste, then the isolates and cells were observed with gram staining. Then it was amplified using a Polymerase Chain Reaction (PCR) instrument with BactF1 and reverse UniB1 primers to obtain the 16s rRNA gene pragment and sequenced to determine the nucleotide base sequence and compared with the bacterial 16s rRNA base sequence registered in the Gene Bank to determine its homology with bacteria. The results of the 16s rRNA base sequence analysis showed that the mustard 1 isolate had a similarity index of 84.65% with Bacillus sp, the gene for the mustard 2 isolate had a similarity index of 84.09% with the Uncultured bacterium clone, and the mustard 3 isolate gene had a similarity index of 85, 42% with Environmental 16s rDNA sequence.

Keywords: lactic acid bacteria-16s rRNA gene fragment-PCR-gram staining-squencing

Topic: Natural Products and Biological Chemistry