Bioactivity Extract of Endophyte Fungi Kalanchoe millotii and Kalanchoe marmorata (Crassulaceae)
Heni Rahmawati (a*), Lutfia Silva Aprina (a), Sari Purbaya (a), Yenny Febriani Yun (a*)

a) Department of Chemistry, Faculty of Science and Informatics, Jenderal Ahmad Yani University, Cimahi 40528
*Henirahmawati2804[at]gmail.com, yenny.febriani[at]lecture.unjani.ac.id

Abstract

Cocor bebek (Kalanchoe millotii & Kalanchoe marmorata) have been many used for cope problem health is one potential as antibacterial and antioxidant. Content metabolites Secondary is also found in microorganisms that live in the body network plants, that is endophyte fungi, Study this aim for knowing species mold isolated endophytes, group metabolites secondary extract mold endophyte as well as its potential as antibacterial and antioxidant. Identification result isolate endophyte fungi from root K. marmorata that is Fusarium Sp., root K. millotii that is Fusarium oxysporum and stem K. millotii that is Penicillium citrinum. Extract ethanol and ethyl acetate endophyte fungi obtained with method fermentation and maceration. Phytochemical test results show that extract the contain metabolites secondary group of alkaloids, flavonoids, steroids, triterpenoids, tannins, and quinones. Test activity antibacterial conducted with disk diffusion method, extract ethanol endophyte fungi F. oxyxporum root K. millotii show potency weak antibacterial to Bacillus subtilis, Escherichia coli and Psedomonas aeruginosa and not potential to Staphylocoocus sureus, while extract ethyl acetate endophyte fungi F. oxyxporum K. millotii root have weak ability to B. subtilis, S.aureus and P. aeruginosa and have moderate ability to bacteria E. coli. Test activity antioxidant conducted with DPPH method, extract ethyl acetate Fusarium oxysporum fungi root K. millotii, extract ethanol Fusarium sp fungi root K. marmorata and Penicillium citrinum fungi stem K. millotii show activity antioxidant category no active and moderate with IC50 647.17 ppm, 577.97 ppm , and 231.73 ppm respectively.

Keywords: Antibacterial, Antioxidant, Kalanchoe marmorata, Kalanchoe millotii, Phytochemical.

Topic: Natural Products and Biological Chemistry