New Isolated Agriculture Waste Bacteria Urease Activity Responds to the Various Buffer Concentration
Nadhifah Astari Putri, Danu Risqi Yudiargo, Fellycita Kartika Afriliona, Fida Alya Putri Rimansa, Frida Ayu Wahyuwati, Izzatul Islami, Karimatul Millah, Endry Nugroho Prasetyo*

Gedung H, Department of Biology, Faculty of Science and Data Analytics, Institut Teknologi Sepuluh Nopember, Kampus ITS, Keputih, Sukolilo, Surabaya 60111, Indonesia
*endry[at]bio.its.ac.id

Abstract

Urease is an enzyme that catalyzes the hydrolysis of urea, which carries out the decomposition of urea into carbon dioxide and ammonia. This enzyme acts as a unique catalyst for its concrete action on urea, thus generating the preferred nitrogen source of many organisms. urease is abundantly found in plants and bacteria. This study aimed to determine the effect of buffer concentration from newly isolated agriculture waste bacteria urease activity. The concentration was set ranging from 10 - 200 mM. Enzymatic activity of urease was conducted by using phosphate buffer. The activity of the enzyme was assayed by the chromoghenic method. Furthermore, the result of this study showed that the best urease activity was achieved using phosphate buffer concentration at 100 mM.

Keywords: Agriculture waste- Bacteria- Buffer concentration- Phosphate buffer- Urease

Topic: Biotechnology