Isolation and Biochemical Screening of CaCO3 Precipitating Bacteria from Malang Southern Coast as An Anticorrosion Agent
Zulkifli, Muhammad Mauludi1- Rozana, Kennis1- Kartikasari, Nuraini1- Prabaningtyas, Sitoresmi1- Witjoro, Agung1- Utomo, Muhammad Andry Prio1*

Biology Department, Faculty of Mathematics and natural Scince, Universitas Negeri Malang

Abstract

Corrosion is a phenomenon which unavoidable in marine environment. Corrosion in steel causes significant losses for marine infrastructure. There are several methods to avoid corrosion. Unfortunately, those methods have negative effect to human and environment health. One of environmentally friendly method that employs calcium carbonate (CaCO3) precipitating bacteria can be an alternative to prevent corrosion since it does not release harmful substance. Therefore, the objectives of this study were to isolating calcium carbonate precipitating bacteria from calcareous beaches in Southern part of Malang and evaluate its specific biochemical characteristics to be applied as anti-corrosion agent. Bacterial isolation was conduct using selective Zobell Marine Agar + CaCl2 media. The biochemical characteristics of the growing colonies that observed were crystal production, biofilm production, and oxygen demand test.

Keywords: CaCO3 precipitating bacteria, biochemical screening, Malang Southern Coast, anticorrosion

Topic: Microbiology