Ethanol Extract of Curcuma xanthorrhiza Rhizome as Hepatoprotector in Rats (Rattus Norvegicus) that CCl4 induced to Malondialdehyde (MDA) Concentration by Thiobarbituric Acid (TBA) Test and Protein Profile by SDS-PAGE.
Hamida Nurlaila1, a), Fahmi Dimas Abdul Azis1, b), and Umi Narsih1, c)

1 Undergraduate of Clinical and Community Pharmacy STIKes Hafshawaty Pesantren Zainul Hasan

Abstract

CCl4 is a xenobiotic compound that could be entered into body by digestion and ingestion system. Its trigger to produce ROS until lipid peroxydation, that is followed by increasing of malondialdehyde (MDA) concentration and cause alteration profile of liver protein band. This research are aimed to determine of malondialdehyde (MDA) concentration and the profile of liver protein band in rats that CCl4 induced. This research used male rats that divided into four groups, that were negative control, positive control (CCl4 induced) and therapy groups (400 and 800 mg/kg BW of Curcuma xanthorrhiza rhizome ethanol extract). Malondialdehyde (MDA) concentration was analyzed by TBA test, its result was 2.042, 3.493, 2.911, and 1.678 mg/L successively. The profile of liver protein band was analyzed by SDS-PAGE, its result was liver protein bands with the molecular weight are 63.55 and 43.88 kDa in positive control was disappear. However, both proteins reappear in therapy groups. Thereby, both 400 and 800 mg/kg BW of Curcuma xanthorrhiza rhizome ethanol extract is able as hepatoprotector which known from decreasing of malondialdehyde (MDA) concentration and reappearance both liver protein bands with the molecular weight are 63.55 and 43.88 kDa.

Keywords: CCl4, ROS, Lipid peroxydation, Curcuma xanthorrhiza Rhizome, Malondialdehyde, Protein Profile, TBA Test, SDS-PAGE.

Topic: Biochemistry