Allicin Promotes Wound Healing In A Diabetic Rats Model By Reducing Inflammation
Agik Priyo Nusantoro (1-2) ,Kuntaman (3), and David Sontani Perdanakusuma(4)

1. Doctoral Program of Medical Science, Faculty of Medicine, Universitas Airlangga, Dr. Moestopo Road No. 47, Surabaya 60285, Indonesia 2. Department of Medical Surgical Nursing, Faculty of Health Science, Universitas Kusuma Husada, Jaya Wijaya Road No. 11, Surakarta 57136, Indonesia
3. Department of Microbiology, Faculty of Medicine, Universitas Airlangga, Dr. Moestopo Road No. 47, Surabaya 60285, Indonesia 4. Department of Reconstructive and Aesthetic Plastic Surgery, Faculty of Medicine, Universitas Airlangga, Dr. Moestopo Road No. 47, Surabaya 60285

Abstract

INTRODUCTION The condition of diabetes mellitus causes postponed wound healing where the inflammatory phase of the wound-healing process becomes prolonged due to excessive inflammatory reactions. Allicin is a natural active substance discovered in garlic with antimicrobial, antioxidant, anticancer, anti-inflammatory, and various benefits. Allicin is often applied to experimental animals to treat certain diseases through intragastric or intraperitoneal administration, but whether Allicin is effectively administered topically as an excision wound medication in a diabetic rat model. The study aimed to see the effect of Allicin provided in a topical form to reduce inflammation and accelerate wound healing. METHOD. Twenty (20) male Wistar rats were modelled for diabetes with Streptozotocin induction. Rats with glucose levels >250mg/dl were considered diabetic rats which were excised in 1cm x 1cm size and divided into two groups. The control group obtained a placebo, and the treatment group received an allicin concentration of 10mg/ml. Both formulas were applied topically once a day for seven days on the excision wound gradually. Both groups were terminated on the third (3rd) and seventh (7th) days to observe inflammatory cells. RESULT AND DISCUSSION. The observation of inflammatory cells used haematoxylin & eosin (H&E) staining on the third (3rd) day in the treatment group with allicin, and the control group with placebo obtained no significant difference. Meanwhile, on the 7th day of post-wound care, the treatment group with allicin and the control group with placebo encountered significant differences in the number of inflammatory cells. The treatment group with allicin revealed a reduction in the number of inflammatory cells. Meanwhile, the control group with placebo presented an improved number of inflammatory cells. CONCLUSION. Allicin applied topically to excision wounds of a diabetic rat model assisted the promotion of the wound-healing process in a diabetic rat m

Keywords: Wound healing, Allicin, Inflammation, Diabetic rats

Topic: Biochemistry