Marine Microalgae DNA Extraction Methods: A Comparative Study
Nuraini Kartikasari (a*), Rifka Fachrunnisa (a), Sheela Chandren(b), Muhammad Andry Prio Utomo(a)

a) Department of Biology, Universitas Negeri Malang, Malang, Indonesia
*nuraini.kartikasari.fmipa[at]um.ac.id
b) Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia, Johor Bahru, Malaysia

Abstract

The recent study of microalgae identification involves molecular research work to facilitate the identification process. It is because identification based on morphological characters is quite difficult due to the similar features of microalgae. There were several methods that can applied to extract the microalgae DNA. However, obtaining DNA from microalgae requires long cultivation times to produce enough cells and many steps are needed for the extraction of DNA, requiring significant time and cost also some cannot be applied for all microalgae species. Therefore, this research aimed to study the optimization of DNA extraction method for different species, protocol simplification, and microalgae and DNA amount.

Keywords: Marine Microalgae, DNA extraction, comparative study

Topic: Microbiology