SCCmec Type III Dominant in Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates From Surgical Wards Inpatients, Dr. Soetomo Hospital Surabaya
Mardiana Lelitawati1,2a), Kuntaman Kuntaman3, Eko Budi Koendhori3, Dewi Santosaningsih4, Aima Insana5

1. Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, Jl. Semarang 5, Malang 65145, Indonesia
2. Department of Microbiology, 2Department of Internal Medicine, Faculty of Medicine, Universitas Airlangga/Dr. Soetomo Hospital Surabaya, Indonesia
3. Department of Microbiology, Faculty of Medicine, Brawijaya University/Dr. Saiful Anwar Hospital, Malang, Indonesia
4. Medical Laboratory Technology, Poltekkes Kemenkes Banjarmasin

Abstract

MRSA strains can grow and spread quickly due to the ability to obtain and utilize mobile genetic elements such as SCCmec. Identification of SCCmec type in a health care centers is important to know the likely source of the spread of MRSA. This study aimed to analyze the distribution of SCCmec type in isolates of MRSA taken from surgical ward inpatients in Dr. Soetomo Hospital, Surabaya. The samples were 30 isolates identified as MRSA colonization in nose and throat from previous studies. Isolates were inoculated onto Mannitol salt agar (MSA) then subcultured onto Brilliance MRSA 2 Agar followed by catalase and coagulase test. 30 DNA template have been amplified with PCR metode for detection of SCCmec type. The result showed that 30/30 isolates (100%) contained SCCmec type III (HA-MRSA). Most of the admission patients in surgical wards were referral patients from secondary hospital so that patients possibly enter the wards already with HA-MRSA colonization. Discharge patients were mostly hospitalized for a long time with the duration of hospitalization was 6 to 132 days with average 35.4 days. Hospitalizations allow the spread of MRSA from patients infected or colonized with MRSA SCCmec type III to healthcare worker or otherwise.

Keywords: Methicillin-resistant Staphylococcus aureus, MRSA, SCCmec type, Surgical Ward, Dr. Soetomo Hospital

Topic: Microbiology